The DNA recognition site for the dioxin-Ah receptor complex. Nucleotide sequence and functional analysis.
The DNA recognition site for the dioxin-Ah receptor complex. Nucleotide sequence and functional analysis.
复制标题
DOI：
--
复制
发表时间：
1988-11
期刊：
影响因子：
--
通讯作者：
M. Denison;J. Fisher;J. Whitlock
中科院分区：
文献类型：
--
作者：
M. Denison;J. Fisher;J. Whitlock
环境污染物2，3，7，8-四氯二苯并-p-二恶英通过与细胞内蛋白质（Ah受体）结合产生其生物学效应。二恶英-Ah受体复合物通过与二恶英响应增强剂相互作用激活细胞色素P1-450基因转录。在这里，我们报告了二恶英-Ah受体复合物与含有“核心”序列5 '-TA/TGCGTG-3'的DNA结合，该序列存在于三种受体依赖性增强子中的每一种中。功能分析表明，配体Ah受体的核心序列的结合不能产生一个积极的增强子和核苷酸侧翼的核心序列必须有助于增强子功能和二恶英的行动。
The environmental contaminant 2,3,7,8-tetra-chlorodibenzo-p-dioxin produces its biological effects by binding to an intracellular protein (the Ah receptor). The dioxin-Ah receptor complex activates cytochrome P1-450 gene transcription by interacting with dioxin-responsive enhancers. Here, we report that the dioxin-Ah receptor complex binds to DNA containing the "core" sequence 5'-TA/TGCGTG-3', which is present in each of three receptor-dependent enhancers. Functional analyses indicate that binding of the liganded Ah receptor to the core sequence fails to generate an active enhancer and that nucleotides flanking the core sequence must contribute to enhancer function and dioxin action.