An integrated microfabricated cell sorter
An integrated microfabricated cell sorter
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DOI：
10.1021/ac0255330
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发表时间：
2002-06-01
影响因子：
7.4
通讯作者：
Quake, SR
中科院分区：
文献类型：
--
作者：
Fu, AY;Chou, HP;Quake, SR
我们研制了一种采用多层软光刻技术的集成微细加工细胞分选机。这种集成的细胞分选机集成了各种微流控功能，包括蠕动泵、阻尼器、开关阀和输入输出井，以协调和自动化的方式执行细胞分选。这种集成式细胞分选机上驱动阀的有效体积可以小到1pl，而光学询问的体积类似于100fl。在这些设备中实现了不同的细胞操作算法，包括细胞捕获。我们还在芯片上演示了对大肠杆菌细胞的分选和回收。
We have developed an integrated microfabricated cell sorter using multilayer soft lithography. This integrated cell sorter is incorporated with various microfluidic functionalities, including peristaltic pumps, dampers, switch valves, and input and output wells, to perform cell sorting in a coordinated and automated fashion. The active volume of an actuated valve on this integrated cell sorter can be as small as 1 pL, and the volume of optical interrogation is similar to100 fL. Different algorithms of cell manipulation, including cell trapping, were implemented in these devices. We have also demonstrated sorting and recovery of Escherichia coli cells on the chip.