BIG regulates stomatal immunity and jasmonate production in Arabidopsis
BIG regulates stomatal immunity and jasmonate production in Arabidopsis
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BIG 调节拟南芥的气孔免疫和茉莉酸产生
DOI：
10.1111/nph.15568
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发表时间：
2019
期刊：
影响因子：
9.4
通讯作者：
Yun-Kuan Liang
中科院分区：
文献类型：
--
作者：
Ruo-Xi Zhang;Shengchao Ge;Jingjing He;Shuangchen Li;Yanhong Hao;Hao Du;Zhongming Liu;Rui Cheng;Yu-Qi Feng;Lizhong Xiong;Li Chuanyou;Alistair M. Hetherington;Yun-Kuan Liang
植物已经进化出一系列的反应，为它们提供保护，免受微生物和其他捕食者的攻击。这些机制中的许多依赖于植物激素茉莉酸（JA）和乙烯（ET）之间的相互作用。本研究通过突变体的基因表达和生理检测来研究拟南芥BIG基因在胁迫反应中的作用，BIG基因的转录被甲基茉莉酸（MeJA）、坏死营养型感染或机械损伤下调。BIG缺乏促进JA依赖性基因诱导，增加JA产生，但限制ET和水杨酸的积累。JA诱导的花青素积累和叶绿素降解增强，气孔免疫力因BIG破坏而受损。细菌和脂多糖（LPS）诱导的气孔关闭在BIG基因突变体中减少，这些突变体对具有不同生活方式的微生物病原体高度敏感，但这些突变体对植食性昆虫的吸引力较小。我们的研究结果表明，BIG负和正调控JA信号通路的MYC 2和ERF 1臂。BIG作为一种新的独特的调节因子，可以调节JA反应，JA和ET的协同作用，以及其他调节拟南芥生长和防御困境的激素相互作用。
Plants have evolved an array of responses that provide them with protection from attack by microorganisms and other predators. Many of these mechanisms depend upon interactions between the plant hormones jasmonate (JA) and ethylene (ET). However, the molecular basis of these interactions is insufficiently understood.Gene expression and physiological assays with mutants were performed to investigate the role of ArabidopsisBIGgene in stress responses.BIGtranscription is downregulated by methyl JA (MeJA), necrotrophic infection or mechanical injury. BIG deficiency promotes JA‐dependent gene induction, increases JA production but restricts the accumulation of both ET and salicylic acid. JA‐induced anthocyanin accumulation and chlorophyll degradation are enhanced and stomatal immunity is impaired by BIG disruption. Bacteria‐ and lipopolysaccaride (LPS)‐induced stomatal closure is reduced inBIGgene mutants, which are hyper‐susceptible to microbial pathogens with different lifestyles, but these mutants are less attractive to phytophagous insects.Our results indicate that BIG negatively and positively regulate the MYC2 and ERF1 arms of the JA signalling pathway. BIG warrants recognition as a new and distinct regulator that regulates JA responses, the synergistic interactions of JA and ET, and other hormonal interactions that reconcile the growth and defense dilemma in Arabidopsis.