Septin function in Candida albicans morphogenesis
Septin function in Candida albicans morphogenesis
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DOI：
10.1091/mbc.e02-01-0013
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发表时间：
2002-08-01
影响因子：
3.3
通讯作者：
Konopka, JB
中科院分区：
文献类型：
--
作者：
Warenda, AJ;Konopka, JB
Septin蛋白在酿酒酵母中的隔膜、交配突起和孢子的形成中起作用，以及在动物细胞中的细胞分裂和其他过程中起作用。白念珠菌septins在这项研究中检查他们的角色，这种多形态，机会致病性真菌，它可以从圆形芽殖酵母拉长菌丝的形态发生。C.白色念珠菌绿色荧光蛋白标记的septin蛋白定位于芽和假菌丝颈部的紧密环，并且在菌丝的新兴芽管中作为更弥散的阵列。缺失分析表明，C. albicans的同源物。酿酒酵母CDC3和CDC12隔膜蛋白是生存力所必需的。与此相反，C.白念珠菌cdc10 Δ和cdc11 Δ突变体是可行的，但显示条件性缺陷的胞质分裂，定位细胞壁几丁质，芽形态。然而，突变体表型并不相同，这表明这些septins具有不同的功能。可行的septin突变体可以刺激进行菌丝形态发生，但形成异常曲率的菌丝，他们不同于野生型在随后的几轮菌丝形成的位点的选择。cdc11Delta突变体在琼脂中包埋时也有侵入性生长缺陷。这些结果进一步扩展了septins的已知作用，证明它们对C.在芽殖和丝状生长期间的白色念珠菌。
The septin proteins function in the formation of septa, mating projections, and spores in Saccharomyces cerevisiae, as well as in cell division and other processes in animal cells. Candida albicans septins were examined in this study for their roles in morphogenesis of this multimorphic, opportunistically pathogenic fungus, which can range from round budding yeast to elongated hyphae. C. albicans green fluorescent protein labeled septin proteins localized to a tight ring at the bud and pseudohyphae necks and as a more diffuse array in emerging germ tubes of hyphae. Deletion analysis demonstrated that the C. albicans homologs of the S. cerevisiae CDC3 and CDC12 septins are essential for viability. In contrast, the C. albicans cdc10Delta and cdc11Delta mutants were viable but displayed conditional defects in cytokinesis, localization of cell wall chitin, and bud morphology. The mutant phenotypes were not identical, however, indicating that these septins carry out distinct functions. The viable septin mutants could be stimulated to undergo hyphal morphogenesis but formed hyphae with abnormal curvature, and they differed from wild type in the selection of sites for subsequent rounds of hyphal formation. The cdc11Delta mutants were also defective for invasive growth when embedded in agar. These results further extend the known roles of the septins by demonstrating that they are essential for the proper morphogenesis of C. albicans during both budding and filamentous growth.