Protein kinases and associated pathways in pluripotent state and lineage differentiation.
Protein kinases and associated pathways in pluripotent state and lineage differentiation.
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多能状态和谱系分化中的蛋白激酶和相关途径。
DOI：
10.2174/1574888x09666140616130217
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发表时间：
2014
影响因子：
2.7
通讯作者：
Ng SW
中科院分区：
文献类型：
--
作者：
Shoni M;Lui KO;Vavvas DG;Muto MG;Berkowitz RS;Vlahos N;Ng SW
蛋白激酶（PKs）介导底物蛋白向磷酸化形式的可逆转化，这是控制细胞内信号转导级联的关键过程。其中，多能性的特征在于特异性表达的PK形成高度互连的调控网络，其最终形成精细平衡的分子开关。目前的高通量磷酸化蛋白质组学方法已经揭示了特定的调节PK及其在控制多能性状态中的功能。多能细胞衍生的内皮和造血发育代表了多能性在癌症治疗和器官再生中的重要性的一个例子。这篇综述试图提供迄今为止已知的激酶谱和PK相关途径的个体特征，调节多能性。阐明潜在的内在和外在信号可以提高我们对不同多能性状态的理解，多能性的维持或诱导，以及定制谱系分化的能力，特别关注用于抗癌治疗的内皮细胞分化，基于细胞的组织工程和再生医学策略。
Protein kinases (PKs) mediate the reversible conversion of substrate proteins to phosphorylated forms, a key process in controlling intracellular signaling transduction cascades. Pluripotency is, among others, characterized by specifically expressed PKs forming a highly interconnected regulatory network that culminates in a finely-balanced molecular switch. Current high-throughput phosphoproteomic approaches have shed light on the specific regulatory PKs and their function in controlling pluripotent states. Pluripotent cell-derived endothelial and hematopoietic developments represent an example of the importance of pluripotency in cancer therapeutics and organ regeneration. This review attempts to provide the hitherto known kinome profile and the individual characterization of PK-related pathways that regulate pluripotency. Elucidating the underlying intrinsic and extrinsic signals may improve our understanding of the different pluripotent states, the maintenance or induction of pluripotency, and the ability to tailor lineage differentiation, with a particular focus on endothelial cell differentiation for anti-cancer treatment, cell-based tissue engineering, and regenerative medicine strategies.