Fibrous nanocrystals of hydroxyapatite loaded with TiO2 nanoparticles for the capture and photocatalytic decomposition of specific proteins
Fibrous nanocrystals of hydroxyapatite loaded with TiO2 nanoparticles for the capture and photocatalytic decomposition of specific proteins
复制标题
DOI：
10.1016/j.colsurfb.2010.03.041
复制
发表时间：
2010-08-01
影响因子：
5.8
通讯作者：
Imai, Hiroaki
中科院分区：
文献类型：
--
作者：
Hirakura, Sho;Kobayashi, Toru;Imai, Hiroaki
用两种沿c轴方向伸长的纤维状晶体观察了溶菌酶（LSZ）和牛血清白蛋白（BSA）在羟基磷灰石（HAp）上的单分子吸附。通过捕获和分解具有特定纳米结构的HAp晶体，实现了特定蛋白质的选择性去除，该纳米结构与锐钛矿型TiO 2相连。成束的短纳米棒。直径为10 nm的纳米棒由于其高比表面积而适合于捕获相对较小的蛋白质分子LSZ，而由于纳米棒之间的间隙窄，相对较大的分子BSA的吸附受到限制。另一方面，大的蛋白质优先吸附到直径为30-60 nm的长纳米针，其在松散聚集的晶体之间具有宽的间隙。在紫外光照射下，捕获的蛋白质被负载在纤维状HAp晶体上的纳米粒子顺利分解。因此，蛋白质分解的光催化活性可以通过在纳米结构HAp晶体表面上的吸附来控制。(C)2010 Elsevier B. V.保留所有权利。
The monomolecular adsorption of lysozyme (LSZ) and bovine serum albumin (BSA) on hydroxyapatite (HAp) was observed by using two types of fibrous crystals elongated in the c-axis. Selective removal of the specific proteins was achieved by the capture and decomposition on the HAp crystals having the particular nanostructures attached with anatase-type TiO2. Bundled short nanorods of ca. 10 nm in diameter were suitable for the capture of a relatively small protein molecule, LSZ, due to their high specific surface area, while the adsorption of a relatively large molecule, BSA, was restricted because of the narrow clearance gap between the nanorods. On the other hand, the large protein preferentially adsorbed to long nanoneedles of 30-60 nm in diameter, which had a wide gap among the loosely aggregated crystals. The captured proteins were smoothly decomposed with anatase nanoparticles loaded on the fibrous HAp crystals under UV irradiation. Thus, the photocatalytic activity for the decomposition of proteins could be controlled with the adsorption on the surface of the nanostructured HAp crystals. (C) 2010 Elsevier B.V. All rights reserved.