Mitosis-promoting factor-mediated suppression of a cloned delayed rectifier potassium channel expressed in Xenopus oocytes
Mitosis-promoting factor-mediated suppression of a cloned delayed rectifier potassium channel expressed in Xenopus oocytes
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DOI：
10.1073/pnas.94.2.537
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发表时间：
1997-01-21
影响因子：
11.1
通讯作者：
Pardo, LA
中科院分区：
文献类型：
--
作者：
Bruggemann, A;Stuhmer, W;Pardo, LA
细胞周期是导致所有细胞类型有丝分裂的关键过程。已知在周期期间许多细胞过程的戏剧性重定向涉及离子通道，改变它们的表达水平或它们的电压依赖性，如在向内整流器的情况下。在这里，我们描述了特异性抑制异源表达的离子通道在非洲爪蟾卵母细胞成熟的开始。在表达大鼠eag（R-eag）钾通道的细胞中，成熟诱导电流幅度急剧降低，在大多数情况下几乎完全降低。卵母细胞成熟的关键分子，促有丝分裂因子（细胞周期蛋白B和p34（cdc 2）的复合物），当注入卵母细胞时，能够诱导类似的变化。
The cell cycle is the crucial process that leads to mitosis in all cell types. The dramatic redirectioning of many cellular processes during the cycle is known to involve ion channels, either changing their level of expression or their voltage dependence, as in the case of inward rectifiers. Here we describe the specific inhibition of heterologously expressed ionic channels at the onset of maturation in Xenopus oocytes. In cells expressing rat eag (R-eag) potassium channels, maturation induces a dramatic reduction in the current amplitude, which is almost complete in most cases. The key molecule in oocyte maturation, the mitosis-promoting factor (a complex of cyclin B and p34(cdc2)), is able to induce similar changes when injected into the oocytes.