Increased light intensity during in vitro culture improves water loss control and photosynthetic performance of Castanea sativa grown in ventilated vessels.
Increased light intensity during in vitro culture improves water loss control and photosynthetic performance of Castanea sativa grown in ventilated vessels.
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DOI：
10.1016/j.scienta.2012.02.005
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发表时间：
2012-05
影响因子：
4.3
通讯作者：
P. Sáez;L. Bravo;M. Latsague;Manuel E. Sánchez;D. Ríos
中科院分区：
文献类型：
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作者：
P. Sáez;L. Bravo;M. Latsague;Manuel E. Sánchez;D. Ríos
评估了离体环境条件、培养容器通风和光照水平对离体培养过程中板栗失水控制和光合能力的影响。苜蓿微芽在通风 (V) 和非通风 (NV) 容器中培养，使用两种光子通量密度 (PFD) 水平，50 和 150μmolphotonsm−2s−1（分别为 L50 和 L150）。相对于传统的苜蓿体外培养（NVL50），通风的使用和辐照度的增加改善了微芽的性能。在 VL150 下生长的微芽显示出气孔密度增加并改善了其功能特性，显示出更椭圆的形状和更低的开放气孔百分比。这与蒸腾速率和气孔导度显着降低相一致。增加光照水平和使用通风容器提高了微芽无害地消散多余吸收能量的能力、水分利用效率和光合作用活性，从而增加了新微芽的产量。体外培养过程中的这些改进产生了具有与幼苗中观察到的解剖和功能特征相似的微芽，这有助于减少体外转移过程中观察到的应激。
The effects of in vitro environmental conditions, ventilation of culture vessels and light level, on water loss control and photosynthetic capacity of Castanea sativa during in vitro culture were assessed. C. sativa microshoots were cultured in ventilated (V) and non ventilated (NV) vessels, using two photon flux density (PFD) levels, 50 and 150μmolphotonsm−2s−1(L50and L150, respectively). The use of ventilation and the increase in irradiance improved the performance of the microshoots with respect to traditional in vitro culture of C. sativa (NVL50). Microshoots grown under VL150showed an increase in stomatal density and improved their functional characteristics, showing a more elliptical shape and lower percentage of open stomata. This paralleled a significantly lower transpiration rate and stomatal conductance. Increasing light level and using ventilated vessels increased the microshoots’ capacity to harmlessly dissipate excess absorbed energy, water use efficiency and photosynthetic activity, resulting in a greater production of new microshoots. These improvements during in vitro culture generate microshoots with anatomical and functional characteristics similar to those observed in seedlings, which could help reduce the stress observed during ex vitro transfer.