Accentuated decrease in social interaction in rats subjected to repeated ethanol withdrawals
Accentuated decrease in social interaction in rats subjected to repeated ethanol withdrawals
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DOI：
10.1097/01.alc.0000023983.10615.d7
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发表时间：
2002-08-01
影响因子：
3.2
通讯作者：
Breese, GR
中科院分区：
文献类型：
--
作者：
Overstreet, DH;Knapp, DJ;Breese, GR
背景：先前的研究表明，反复退出慢性乙醇暴露可以点燃啮齿动物癫痫发作。在这篇文章中，一个三周期模型的乙醇暴露和撤退的社会互动测试的anxiety.Methods：大鼠暴露于乙醇（7%或4.5%）的饮食在3个周期的5天，与2天的周期之间的撤退。之间5和6小时后，乙醇被删除，对大鼠被放置在开放的领域商会的社会互动行为和运动activity.Results：评估后，第三个周期的乙醇（7%）介绍，男性和女性大鼠表现出较低的社会互动行为（更多的焦虑）和活动比后一个单一的周期。大鼠暴露于相似量的乙醇，但在乙醇仍然可用时进行测试，并没有表现出社会互动的减少。社会互动的减少仍然存在长达24小时，但在乙醇撤出后48小时消失。当大鼠被允许8或16天从三周期方案的影响中恢复时，进一步暴露于5天的7%乙醇饮食导致与三周期方案相似的戒断时社会互动减少。与此相反，大鼠连续暴露于7%的乙醇饮食连续15天表现出较高水平的社会互动时，保持在控制饮食8或16天，然后再次暴露于乙醇。暴露于三个周期的协议，并允许32天恢复之前，再次暴露于乙醇的大鼠仍然有部分赤字的社会互动。最后，动物反复退出4.5%的乙醇表现出减少社会互动没有改变活动后，最终退出乙醇，而大鼠连续暴露于4.5%的乙醇饮食没有表现出减少社会互动或活动。无论是血液中的乙醇浓度，也不改变体重可以解释这些行为differentiation.Conclusion：反复退出乙醇可以导致加重或更持久的焦虑样行为的大鼠，减少社会交往。戒断引起的自发活动减少并不因反复戒断而加重。这种反复从乙醇中提取的模型可能有助于定义这种加重的神经化学基础。
Background: Previous work has shown that repeated withdrawals from chronic ethanol exposure can kindle seizures in rodents. In this article, the effects of a three-cycle model of ethanol exposure and withdrawal on the social interaction test of anxiety are summarized.Methods: Rats were exposed to ethanol (7% or 4.5%) diets over three periods of 5 days, with 2 days of withdrawal between cycles. Between 5 and 6 hr after the ethanol was removed, pairs of rats were placed in open field chambers for the assessment of social interaction behavior and locomotor activity.Results: After the third cycle of ethanol (7%) presentation, both male and female rats exhibited lower social interaction behavior (more anxiety) and activity than after a single cycle. Rats exposed to a similar amount of ethanol but tested while ethanol was still available did not exhibit a reduction in social interaction. The decrease in social interaction was still present for up to 24 hr but had disappeared by 48 hr after ethanol was withdrawn. When rats were allowed 8 or 16 days to recover from the effects of the three-cycle protocol, a further exposure to 5 days of 7% ethanol diet resulted in a reduction in social interaction on withdrawal similar to that seen from the three-cycle protocol. In contrast, rats exposed continuously to 7% ethanol diet for 15 consecutive days exhibited higher levels of social interaction when maintained on control diet for 8 or 16 days and then reexposed to ethanol. Rats that were exposed to the three-cycle protocol and allowed 32 days to recover before being reexposed to ethanol still had a partial deficit in social interaction. Finally, animals subjected to repeated withdrawals from 4.5% ethanol exhibited a reduction in social interaction without a change in activity after the final withdrawal from ethanol, whereas rats exposed continuously to a 4.5% ethanol diet did not exhibit a reduction in social interaction or activity. Neither blood ethanol concentrations nor changes in body weight could account for these behavioral differences.Conclusion: Repeated withdrawal from ethanol can lead to accentuated or more persistent anxiety-like behavior in rats, as indicated by a decrease in social interaction. The withdrawal-induced decrease in locomotor activity is not accentuated by repeated withdrawals. This model of repeated withdrawals from ethanol may prove useful in defining the neurochemical basis of this accentuation.