Identification and characterization of a novel secreted antigen 1 of Babesia microti and evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test
Identification and characterization of a novel secreted antigen 1 of Babesia microti and evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test
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DOI：
10.1016/j.parint.2010.11.001
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发表时间：
2011-06-01
影响因子：
1.9
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Luo, Yuzi;Jia, Honglin;Xuan, Xuenan
在这里，我们确定了一个新的分泌抗原命名为Bababelium microti分泌抗原1（BmSA 1）的免疫筛选一个B。用含B.感染microti的仓鼠。在用重组BmSA 1（rBmSA 1）免疫的小鼠中产生的抗体识别天然33-kDa寄生虫蛋白。rBmSA 1的酶联免疫吸附试验（ELISA）检测到特异性抗体，早在6和4天后，从实验感染B的仓鼠血清感染。田鼠Gray株（US型）和实验感染B. microti慕尼黑菌株（啮齿动物分离株）。此外，使用rBmSA 1的快速免疫层析试验（ICT）在实验感染B的仓鼠中检测到特异性抗体。从感染后第6天到至少第270天，感染的感染细胞中没有任何一种被感染，这与ELISA的结果非常一致。此外，对日本首例人巴贝斯虫病（科比型）患者血清的分析表明，使用rBmSA 1通过ELISA可在患者和阳性供体中检测到特异性抗体，ICT结果与ELISA数据一致。综上所述，这些结果表明，BmSA 1可能是一个有前途的和通用的目标，发展ELISA和ICT的血清诊断人类巴贝虫病和流行病学调查的啮齿动物水库。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Here, we identified a novel secreted antigen designated as Babesia microti secreted antigen 1 (BmSA1) by immunoscreening a B. microti cDNA expression library using the sera from hamsters immunized with plasma, putatively containing secreted antigens, from B. microti-infected hamsters. Antibodies raised in mice immunized with recombinant BmSA1 (rBmSA1) recognized a native 33-kDa parasite protein. An enzyme-linked immunosorbent assay (ELISA) of rBmSA1 detected specific antibodies as early as 6 and 4 days post-infection in sera from a hamster experimentally infected with B. microti Gray strain (US type) and a mouse experimentally infected with B. microti Munich strain (rodent isolate), respectively. Moreover, a rapid immunochromatographic test (ICT) using rBmSA1 detected specific antibodies in a hamster experimentally infected with B. microti from day 6 to at least day 270 post-infection, which was quite consistent with the results of the ELISA. In addition, analysis of the sera involved in the first case of human babesiosis in Japan (Kobe type) showed that specific antibodies were detectable in the patient and the positive donor by ELISA using rBmSA1, and the ICT result was identical to the ELISA data. Taken together, these results indicated that BmSA1 could be a promising and universal target for developing both ELISA and ICT for the serodiagnosis of human babesiosis and for an epidemiological survey of its rodent reservoir. (C) 2010 Elsevier Ireland Ltd. All rights reserved.