Comparative effects of interleukin-12 and interleukin-4 on cytokine responses by antigen-stimulated memory CD4+ T cells of cattle: IL-12 enhances IFN-gamma production, whereas IL-4 has marginal effects on cytokine expression.
Comparative effects of interleukin-12 and interleukin-4 on cytokine responses by antigen-stimulated memory CD4+ T cells of cattle: IL-12 enhances IFN-gamma production, whereas IL-4 has marginal effects on cytokine expression.
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IL-12 和 IL-4 对抗原刺激的牛记忆 CD4 T 细胞的细胞因子反应的影响比较：IL-12 增强 IFN-γ 的产生，而 IL-4 对细胞因子表达的影响有限。
DOI：
10.1089/107999099313587
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Brown,WC
中科院分区：
文献类型：
--
作者：
Tuo,W;Estes,DM;Brown,WC
Interleukin-12 (IL-12) and IL-4 are important immunoregulatory cytokines that determine the fate of naive T cells during antigen priming in mice and also influence cytokine synthesis by differentiated murine and human T cells. The roles of these cytokines in regulating the differentiation and effector function of bovine T cells are less well studied. We investigated the ability of human IL-12 and bovine IL-4 to modify cytokine expression by antigen-stimulated T cells from cattle immune to the protozoal parasites Babesia bovis and Babesia bigemina or reactive with Mycobacterium bovis purified protein derivative. Peripheral blood mononuclear cells (PBMC) were cultured with specific antigen and IL-4 or IL-12 for 1 week. Then viable lymphoblasts consisting of predominantly CD4+ T cells were restimulated with antigen and antigen-presenting cells (APC) with or without cytokine. Cell lines were cultured for several weeks, and following restimulation with antigen and APC in the absence of exogenous cytokine, the cell lines were analyzed for proliferation, interferon-gamma (IFN-gamma) production, and expression of IL-2, IL4-, IL-10, or IFN-gamma transcript levels using a quantitative competitive RT-PCR. IL-12 and IL-4 had no effect on the composition of CD4, CD8, or gamma delta T cells in the cell lines or on the level of antigen-induced proliferation. IL-12 stimulated enhanced levels of IFN-gamma protein and transcript expression in all cell lines, with no consistent effects on IL-2 or IL-4 expression. In two B. bovis-specific cell lines, IL-12 suppressed IL-10 expression. IL-4 had no consistent effect on expression of any cytokine. These results indicate the use of IL-12 as an adjuvant to enhance type 1 cytokine responses in cattle during antigen priming.
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人类过敏原特异性 Th2 细胞中缺乏 IL-12 信号传导。
影响因子：
4.4
作者：
C. Hilkens;Gerald Messer;K. Tesselaar;A. G. I. V. Rietschoten;M. Kapsenberg;E. Wierenga
通讯作者：
E. Wierenga
白细胞介素 4 抑制由辅助细胞依赖性受体参与刺激的幼稚 T 细胞产生白细胞介素 2 和干扰素 γ。
DOI：
--
发表时间：
1993
影响因子：
11.1
作者：
Toshio Tanaka;J. Hu‐Li;R. Seder;B. Groth;W. Paul
通讯作者：
W. Paul
DOI：
10.1073/pnas.89.13.6065
发表时间：
1992-07-01
影响因子：
11.1
作者：
HSIEH, CS;HEIMBERGER, AB;MURPHY, KM
通讯作者：
MURPHY, KM
IL-12 对体内细胞因子基因表达和 Ig 同种型选择的影响。
影响因子：
4.4
作者：
S. Morris;K. Madden;Jeffrey J. Adamovicz;W. Gause;Brian R. Hubbard;Maurice K. Gately;F. Finkelman
通讯作者：
F. Finkelman
影响因子：
5.4
作者：
KENNEDY, MK;PICHA, KS;GRABSTEIN, KH
通讯作者：
GRABSTEIN, KH