Effect of adrenalectomy on transport in the rat medullary thick ascending limb.
Effect of adrenalectomy on transport in the rat medullary thick ascending limb.
复制标题
肾上腺切除术对大鼠髓质粗升肢运输的影响。
DOI：
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发表时间：
1987
影响因子：
15.9
通讯作者：
R. Jamison
中科院分区：
文献类型：
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作者：
J. Work;R. Jamison
先前对肾上腺切除 (Adx) 大鼠的研究表明，醛固酮可能调节海伦环上升部分的离子转运。为了直接检查肾上腺切除术对运输的影响，从Adx中分离髓质厚升肢（Mtal）节段，用醛固酮替换Adx（Adx + Ald，0.5微克X 100克X体重X天），并对照Sprague-Dawley大鼠。与对照组或 Adx + Ald 组相比，Adx 大鼠 Mtal 节段的净钠通量和净氯通量均显着减少。 Adx + Ald 组中外源醛固酮的生理水平增加了净氯化钠通量，接近对照值。三组之间的净钾通量没有差异。我们得出的结论是，肾上腺切除术会损害 Mtal 中的 NaCl 重吸收，但不会损害 K 转运，并且醛固酮可以恢复这一过程。这种重吸收缺陷可能导致与肾上腺功能不全相关的尿液浓缩和稀释异常。
Previous studies in adrenalectomized (Adx) rats suggest that aldosterone may regulate ion transport in the ascending portion of Helen's loop. In order to examine directly the effect of adrenalectomy on transport, medullary thick ascending limb (Mtal) segments were isolated from Adx, Adx replaced with aldosterone (Adx + Ald, 0.5 micrograms X 100 g X body wt X d), and control Sprague-Dawley rats. Both net sodium and net chloride fluxes were significantly less in the Mtal segments from Adx rats compared with those in the control or Adx + Ald group. Physiologic levels of exogenous aldosterone increased net sodium chloride flux toward control values in the Adx + Ald group. Net potassium flux was not different among the three groups. We conclude that adrenalectomy impairs reabsorptive NaCl but not K transport in the Mtal, and that aldosterone restores this process. This reabsorptive defect may contribute to the urinary concentrating and diluting abnormality associated with adrenal insufficiency.