Genetic variation among temporally and geographically distinct West Nile virus isolates, United States, 2001, 2002.
Genetic variation among temporally and geographically distinct West Nile virus isolates, United States, 2001, 2002.
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DOI：
10.3201/eid0911.030301
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发表时间：
2003-11
影响因子：
11.8
通讯作者：
Barrett, Alan D T
中科院分区：
文献类型：
--
作者：
Davis, C Todd;Beasley, David W C;Guzman, Hilda;Raj, Rushker;D'Anton, Mary;Novak, Robert J;Unnasch, Thomas R;Tesh, Robert B;Barrett, Alan D T
2001年和2002年夏季和秋季收集的22株西尼罗河病毒（WNV）分离株的部分核苷酸序列分析表明，在美国不同地理区域传播的菌株之间存在遗传变异，并在1999年和2000年期间从美国东北部收集的分离株中继续发散。2，004个核苷酸区域的序列分析表明，14个分离物共享两个核苷酸突变和一个氨基酸取代时，它们与原型WN-NY 99株相比，其中10个分离物共享一个额外的核苷酸突变。相比之下，从德克萨斯州东南部沿海地区收集的分离株与WN-NY 99有以下差异：5个核苷酸突变和1个氨基酸取代。22株WN-NY 99分离株间的核苷酸差异最大为0.35%（平均为0.18%）。这些结果显示了遗传相似的西尼罗河病毒分离株的地理聚类，以及2002年期间可能出现的在美国大部分地区流行的显性变异。
Analysis of partial nucleotide sequences of 22 West Nile virus (WNV) isolates collected during the summer and fall of 2001 and 2002 indicated genetic variation among strains circulating in geographically distinct regions of the United States and continued divergence from isolates collected in the northeastern United States during 1999 and 2000. Sequence analysis of a 2,004-nucleotide region showed that 14 isolates shared two nucleotide mutations and one amino acid substitution when they were compared with the prototype WN-NY99 strain, with 10 of these isolates sharing an additional nucleotide mutation. In comparison, isolates collected from coastal regions of southeast Texas shared the following differences from WN-NY99: five nucleotide mutations and one amino acid substitution. The maximum nucleotide divergence of the 22 isolates from WN-NY99 was 0.35% (mean = 0.18%). These results show the geographic clustering of genetically similar WNV isolates and the possible emergence of a dominant variant circulating across much of the United States during 2002.