THE N-TERMINAL REGION OF THE ALPHA-SUBUNIT OF THE TRAP COMPLEX HAS A CONSERVED CLUSTER OF NEGATIVE CHARGES
THE N-TERMINAL REGION OF THE ALPHA-SUBUNIT OF THE TRAP COMPLEX HAS A CONSERVED CLUSTER OF NEGATIVE CHARGES
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DOI：
10.1016/0014-5793(94)00693-8
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发表时间：
1994-08-08
期刊：
影响因子：
3.5
通讯作者：
PREHN, S
中科院分区：
文献类型：
--
作者：
HARTMANN, E;PREHN, S
TRAP复合体的α亚基(TRAPα)是内质网(ER)的一种单跨膜蛋白，发现于跨膜转运的新生多肽链附近。在这里，我们证明了陷阱α广泛存在于真核生物中，正如它在人、鱼和植物中的存在所表明的那样。尽管序列同源性远低于易位位点上的其他蛋白质，但总体拓扑结构、糖基化位点的位置以及最有趣的是电荷分布都是保守的。这些数据表明，圈闭复合体具有普遍的功能。
The alpha-subunit of the TRAP complex (TRAP alpha) is a single-spanning membrane protein of the endoplasmic reticulum (ER) which is found in proximity of nascent polypeptide chains translocating across the membrane. Here, we demonstrate the widespread occurrence of TRAP alpha in eukaryotes as indicated by its existence in man, fish and plants. Despite the fact that the sequence homology is much lower than for other proteins in the translocation site, the overall topology, the location of the glycosylation sites and, most interestingly, the distribution of charges are conserved. These data indicate that the TRAP complex has a ubiquitous function.