From barcodes to genomes: extending the concept of DNA barcoding
From barcodes to genomes: extending the concept of DNA barcoding
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DOI：
10.1111/mec.13549
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发表时间：
2016-04-01
影响因子：
4.9
通讯作者：
Taberlet, Pierre
中科院分区：
文献类型：
--
作者：
Coissac, Eric;Hollingsworth, Peter M.;Taberlet, Pierre
DNA条形码对生物多样性科学产生了重大影响。为几个条形码基因座建立大规模数据库的优雅简单性正在继续改变我们对物种多样性模式的理解，并继续提高人类区分物种的能力。利用下一代测序技术的发展和基因组测序成本的降低，现在有机会将DNA条形码概念扩展到新类型的基因组数据。我们说明了这样做的好处和能力，并指出在真正可扩展之前需要克服的限制和障碍。我们提倡双轨方法：（i）继续并加速全球努力，使用标准DNA条形码建立地球上生命的DNA条形码参考文库，（ii）积极开发和应用扩展DNA条形码，使用基因组略读来增强标准条形码方法。
DNA barcoding has had a major impact on biodiversity science. The elegant simplicity of establishing massive scale databases for a few barcode loci is continuing to change our understanding of species diversity patterns, and continues to enhance human abilities to distinguish among species. Capitalizing on the developments of next generation sequencing technologies and decreasing costs of genome sequencing, there is now the opportunity for the DNA barcoding concept to be extended to new kinds of genomic data. We illustrate the benefits and capacity to do this, and also note the constraints and barriers to overcome before it is truly scalable. We advocate a twin track approach: (i) continuation and acceleration of global efforts to build the DNA barcode reference library of life on earth using standard DNA barcodes and (ii) active development and application of extended DNA barcodes using genome skimming to augment the standard barcoding approach.