High frequency plant regeneration from mature embryo explants of highland barley (Hordeum vulgare L. var. nudum Hk. f.) under endosperm-supported culture
High frequency plant regeneration from mature embryo explants of highland barley (Hordeum vulgare L. var. nudum Hk. f.) under endosperm-supported culture
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胚乳支持培养下青稞 (Hordeum vulgare L. var. nudum Hk. f.) 成熟胚外植体的高频植物再生
DOI：
10.1007/s11240-008-9437-2
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发表时间：
2008-11-01
影响因子：
3
通讯作者：
Jia, J. F.
中科院分区：
文献类型：
--
作者：
He, T.;Jia, J. F.
以青稞成熟胚为外植体，建立了一套高效的离体植株再生体系。变种努杜姆港f.）在胚乳支持的培养条件下。从成熟种子中切下具有胚乳的胚（胚乳支持培养物，ES）或不具有胚乳的胚（非胚乳支持培养物，内斯），并在补充有各种浓度的2，4-D（1-5 mg l-1）的MS培养基上培养以诱导愈伤组织。ES外植体的愈伤组织诱导率显著低于内斯外植体（P0.05）.内斯外植体在MS +3 mg l−1 2，4-D培养基上愈伤组织诱导率最高（97.6%）。当初级愈伤组织在降低浓度的2，4-D（0.5 mg l-1）下维持3周时，形成胚性愈伤组织。然后将胚性愈伤组织转移到补充有不同浓度的BA（1-5 mg l-1）和500 mg l-1酪蛋白水解物（CH）的MS培养基中用于芽再生。但ES细胞诱导的愈伤组织再生能力显著高于内斯诱导的愈伤组织（P0.05）。在含有2 mg l-1 BA的MS培养基上，从ES+衍生的愈伤组织观察到最佳反应（81.3%）。将具有发达根系的再生植株转移到盆中，在盆中它们生长良好，达到成熟并产生可育种子。该方法可用于遗传操作研究。
An in vitro protocol for efficient plant regeneration has been developed from mature embryo explants of highland barley (Hordeum vulgare L. var. nudum Hk. f.) under endosperm-supported culture. Embryos with (endosperm-supported culture, ES) or without endosperm (non-endosperm-supported culture, NES) were excised from mature seeds and cultured on MS medium supplemented with various concentrations of 2,4-D (1–5 mg l−1) for callus induction. The percentage of callus induction from ES explants was significantly (P < 0.05) lower than that from NES. The highest frequency (97.6%) of callus induction was obtained from NES explants on MS medium containing 3 mg l−1 2,4-D. When the primary calli were maintained at a reduced concentration of 2,4-D (0.5 mg l−1) for 3 weeks, embryogenic calli were formed. The embryogenic calli were then transferred to MS medium supplemented with different concentrations of BA (1–5 mg l−1) and 500 mg l−1 casein hydrolysate (CH) for shoot regeneration. However, the capacity of plant regeneration from ES explant-derived calli was significantly (P < 0.05) higher than that from NES. The best response (81.3%) was observed from ES explant-derived calli on MS medium containing 2 mg l−1 BA. Regenerated plantlets with well-developed root systems were transferred to pots where they grew well, attained maturity and produced fertile seeds. This method could be employed for genetic manipulation studies.