Molecular characterization of stress resistance-related chitinase genes of Brassica rapa
Molecular characterization of stress resistance-related chitinase genes of Brassica rapa
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DOI：
10.1016/j.plaphy.2012.06.015
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发表时间：
2012-09-01
影响因子：
6.5
通讯作者：
Nou, Ill-Sup
中科院分区：
文献类型：
--
作者：
Ahmed, Nasar Uddin;Park, Jong-In;Nou, Ill-Sup
甘蓝型油菜是世界范围内一个重要的蔬菜类群，受到生物和非生物胁迫的影响。分子生物学技术为解决这些问题提供了最有效的方法。诱导植物的防御反应包括病程相关蛋白的产生，而几丁质酶是非常重要的PR蛋白。我们收集了30个类几丁质酶基因，其中3个是从我们的白菜全长cDNA文库中获得的。OSome和27个来自Brassica数据库。序列分析和比较研究证实，它们都是I-V和VII类几丁质酶基因。这些基因也显示出与其他生物抗逆性相关的植物几丁质酶高度同源。观察到这些基因的器官特异性表达，其中有7个基因在感染尖孢镰刀菌TSP后表现出显著的反应。甘蓝中的粘附素和大白菜中的16个基因在非生物胁迫处理后表现出响应表达。BrCLP1、8、10、17和18在生物胁迫和非生物胁迫处理后反应普遍，表现出较高的潜力。综上所述，这些结果表明这些几丁质酶基因可能是抗逆性甘蓝型油菜的有用资源。(C)2012年爱思唯尔·马森公司。版权所有。
Brassica is an important vegetable group worldwide that is impacted by biotic and abiotic stresses. Molecular biology techniques offer the most efficient approach to address these concerns. Inducible plant defense responses include the production of pathogenesis-related (PR) proteins, and chitinases are very important PR proteins. We collected 30 chitinase like genes, three from our full-length cDNA library of Brassica rapa cv. Osome and 27 from Brassica databases. Sequence analysis and comparison study confirmed that they were all class I-V and VII chitinase genes. These genes also showed a high degree of homology with other biotic stress resistance-related plant chitinases. An organ-specific expression of these genes was observed and among these, seven genes showed significant responses after infection with Fusarium oxysporum tsp. conglutinans in cabbage and sixteen genes showed responsive expression after abiotic stress treatments in Chinese cabbage. BrCLP1, 8, 10, 17 and 18 responded commonly after biotic and abiotic stress treatments indicating their higher potentials. Taken together, the results presented herein suggest that these chitinase genes may be useful resources in the development of stress resistant Brassica. (C) 2012 Elsevier Masson SAS. All rights reserved.