Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota
Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota
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DOI：
10.1093/femsec/fiy002
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发表时间：
2018-02-01
影响因子：
4.2
通讯作者：
Moya, Andres
中科院分区：
文献类型：
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作者：
Rosas, Tania;Garcia-Ferris, Carlos;Moya, Andres
真核生物与微生物建立了共生关系，这使它们能够完成它们单独无法完成的功能。在德国小蠊（Blattella germanica）中，专性内共生体Blattabacterium与丰富的肠道微生物群共存。Blattabacterium的传播是垂直的，但对肠道微生物群如何在新生儿个体中定植知之甚少。在这项研究中，我们治疗了B。在两代期间用利福平（一种广谱抗生素）处理德国人群体，并分析了对照和利福平处理的群体中的肠道细菌组成和Blattabacterium负荷。利福平对肠道微生物群组成产生了强烈的影响，在没有将抗生素添加到饮食中的情况下，肠道微生物群组成在第二代中恢复。此外，我们观察到饮食中，特别是粪便中存在的细菌种类对建立肠道微生物群做出了显着贡献。最后，Blattabacterium种群在第一代期间不受成人抗生素治疗的影响，但在第二代中强烈减少，表明这种细胞内共生体仅在成熟卵母细胞感染期间对利福平敏感，当它处于细胞外阶段时。
Eukaryotes have established symbiotic relationship with microorganisms, which enables them to accomplish functions that they cannot perform alone. In the German cockroach, Blattella germanica, the obligate endosymbiont Blattabacterium coexists with a rich gut microbiota. The transmission of Blattabacterium is vertical, but little is known about how the gut microbiota colonizes newborn individuals. In this study, we treated B. germanica populations with rifampicin, a broad-spectrum antibiotic, during two generations and analyzed gut bacterial composition and the Blattabacterium load in control and rifampicin-treated populations. Rifampicin exerted a drastic effect on gut microbiota composition, which recovered in the second generation in the case where the antibiotic was not added to the diet. Furthermore, we observed that bacterial species present in the diet, and particularly in the feces, contribute significantly to establishing the gut microbiota. Finally, the Blattabacterium population remained unaffected by the antibiotic treatment of adults during the first generation but was strongly reduced in the second generation, suggesting that this intracellular symbiont is sensitive to rifampicin only during the infection of the mature oocytes, when it is in an extracellular stage.