MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein
MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein
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MOV10将甲型流感病毒的RNP隔离在细胞质中并被病毒NS1蛋白拮抗
DOI：
10.1042/bcj20180754
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发表时间：
2019-02-14
影响因子：
4.1
通讯作者：
Guo, Fei
中科院分区：
文献类型：
--
作者：
Li, Jian;Hu, Siqi;Guo, Fei
MOV10已成为一种重要的宿主抗病毒因子。MOV10不仅抑制包括人类免疫缺陷病毒1型、丙型肝炎病毒和水泡性口炎病毒在内的多种病毒，而且还限制反转录元件长分布核苷酸元件-1、Alu、SVA和脑池内A颗粒的活性。在这里，我们报道了MOV10通过与病毒核蛋白(NP)相互作用，将病毒RNP隔离在细胞质中，导致病毒vRNA降解来抑制甲型流感病毒的感染。MOV10的抗病毒活性依赖于P小体的完整性。我们还发现，MOV10的抗病毒活性部分地被病毒NS1蛋白所抵消，NS1蛋白干扰了MOV10与病毒NP的相互作用，并通过溶酶体途径导致MOV10的降解。此外，NS1缺陷的甲型流感病毒更容易受到MOV10的限制。我们的数据不仅扩展了MOV10的抗病毒谱，而且揭示了NS1蛋白是MOV10的第一个病毒拮抗剂。
MOV10 has emerged as an important host antiviral factor. MOV10 not only inhibits various viruses, including human immunodeficiency virus type 1, hepatitis C virus and vesicular stomatitis virus, but also restricts the activity of retroelements long interspersed nucleotide element-1, Alu, SVA and intracisternal A particles. Here, we report that MOV10 suppresses influenza A virus infection through interacting with viral nucleoprotein (NP), sequestering viral RNP in the cytoplasm and causing the degradation of viral vRNA. The antiviral activity of MOV10 depends on the integrity of P-bodies. We also found that the antiviral activity of MOV10 is partially countered by viral NS1 protein that interferes with the interaction of MOV10 with viral NP and causes MOV10 degradation through the lysosomal pathway. Moreover, NS1-defective influenza A virus is more susceptible to MOV10 restriction. Our data not only expand the antiviral spectrum of MOV10 but also reveal the NS1 protein as the first viral antagonist of MOV10.