Evaluating the expression level of miR-9-5p and miR-192-5p in gastrointestinal cancer: introducing novel screening biomarkers for patients
Evaluating the expression level of miR-9-5p and miR-192-5p in gastrointestinal cancer: introducing novel screening biomarkers for patients
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DOI：
10.1186/s13104-020-05071-9
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发表时间：
2020-04-19
影响因子：
1.8
通讯作者：
Faghihloo, Ebrahim
中科院分区：
文献类型：
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作者：
Tavakolian, Shaian;Goudarzi, Hossein;Faghihloo, Ebrahim
目的研究表明，环境、遗传等多种因素与肿瘤的发生密切相关，其中包括microRNA的异常表达。已经在几项研究中确定了microRNA在调节胃肠道癌症中多种信号通路中的关键作用。在这项研究中，我们的目的是评估microRNA-9和-192在结肠癌和胃癌中的表达。从结肠癌和胃癌患者的组织和血清样品中提取RNA后，合成cDNA。然后通过实时荧光定量PCR检测miR-9- 5 p和miR-192- 5 p的表达水平。结果胃癌组织中miR-9- 5 p的相对表达量明显低于癌旁正常组织（P < 0.05），而结肠癌组织中miR-9 - 5 p的相对表达量无明显变化。与这一发现一致，我们还发现，与正常胃组织相比，胃癌组织中miR-192- 5 p的表达水平降低。鉴于胃癌中miR-9- 5 p和miR-192- 5 p表达水平的降低，可以假定将这些miRNA视为有希望的诊断生物标志物。
Objective It has been indicated that there is a tight association between cancer and different factors, such as environment and genetics, including aberrantly expressed microRNAs. The crucial role of microRNAs in the regulation of diverse signaling pathways in gastrointestinal cancer has been established in several studies. In this study, we aimed to evaluate the expression of microRNA-9 and -192 in colon and gastric cancers. After extracting the RNA from tissues and serum samples of patients, suffering from colon and gastric cancer, cDNA was synthesized. Then by performing quantitative real-time PCR, we evaluated the expression level of miR-9-5p and miR-192-5p in collected samples. Results Unlike to colon cancer in which the expression level of miR-9-5p remained unchanged, the relative expression of this miRNA decreased remarkably in gastric cancer (with P value < 0.05), in comparison with normal adjacent tissues. In agreement with this finding, we also found that the expression level of miR-192-5p was decreased in gastric cancer tissues, compared to normal gastric tissue. Given the reduction in the expression level of miR-9-5p and miR-192-5p in gastric cancer, it could be postulated to consider these miRNAs as promising diagnostic biomarkers.