Production of multiple cytokines by cultured human melanomas.
Production of multiple cytokines by cultured human melanomas.
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DOI：
10.1111/j.1600-0625.1993.tb00031.x
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发表时间：
1993-08-01
影响因子：
3.6
通讯作者：
Guerry, D 4th
中科院分区：
文献类型：
--
作者：
Bennicelli, J L;Guerry, D 4th
我们筛选了8个原代黑色素瘤细胞系和21个转移性黑色素瘤细胞系，用于细胞因子的结构性分泌。黑色素瘤对转化生长因子-β(8/25株)和干扰素(7/12株)有生物活性，但对IL-2无活性。免疫分析检测IL-1α(4/25)、IL-1β(12/25)、IL-6(13/29)、IL-8(29/29)、转化生长因子-β2(5/12)和GM-CSF(11/29)，但未检测到IL-3、IL-4、肿瘤坏死因子-α和干扰素-γ。细胞因子的产生与原发和转移性疾病的细胞培养没有明显的关联，而且所有被测试的细胞系都只产生IL-8。这些数据表明，培养的黑色素瘤产生多种细胞因子，这些细胞因子可能会影响体内肿瘤的生长。
We screened a panel of 8 primary and 21 metastatic melanoma cell lines for constitutive secretion of cytokines. Melanomas expressed bioactivity for TGF-beta (8/25 lines) and IFN (7/12), but not IL-2. Immunoassays detected IL-1 alpha (4/25), IL-1 beta (12/25), IL-6 (13/29), IL-8 (29/29), TGF-beta 2 (5/12) and GM-CSF (11/29), but not IL-3, IL-4, TNF-alpha, or IFN-gamma. There was no preferential association of cytokine production with cells cultured from primary versus metastatic disease, and only IL-8 was produced by all lines tested. These data demonstrate that cultured melanomas produce a variety of cytokines which are potentially capable of influencing tumor growth in vivo.