Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway
Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway
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DOI：
10.1080/09168451.2015.1136881
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发表时间：
2016-02
期刊：
影响因子：
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通讯作者：
D. Yamada;K. Kawahara;M. Ozaki;T. Maeda
中科院分区：
文献类型：
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作者：
D. Yamada;K. Kawahara;M. Ozaki;T. Maeda
我们发现来源于刘易斯肺癌细胞的条件培养基下调了成骨样MC 3 T3-E1细胞中Semaphorin 3a（Sema 3a）mRNA的表达，并增加了雷帕霉素复合物1（mTORC 1）的活性。此外，雷帕霉素抑制mTORC 1抵消了条件培养基对Sema 3a mRNA表达的影响。这些结果表明肿瘤细胞以mTORC 1依赖的方式降低成骨细胞中Sema 3a mRNA的表达。
We found that conditioned medium derived from Lewis Lung Carcinoma cells down-regulated Semaphorin3a (Sema3a) mRNA expression and increased the activity of mammalian target of rapamycin complex 1 (mTORC1) in osteoblast-like MC3T3-E1 cells. Furthermore, mTORC1 inhibition with rapamycin counteracted the effect of conditioned media on Sema3a mRNA expression. These results suggest that tumor cells decrease Sema3a mRNA expression in osteoblast in an mTORC1-dependent manner.