High expression of macrophage migration inhibitory factor in the murine lacrimal gland.
High expression of macrophage migration inhibitory factor in the murine lacrimal gland.
复制标题
小鼠泪腺中巨噬细胞迁移抑制因子的高表达。
DOI：
10.1097/ico.0b013e3181c06466
复制
发表时间：
2010
期刊：
影响因子：
2.8
通讯作者：
Chuck,RoyS
中科院分区：
文献类型：
--
作者：
Park,ChoulYong;Shin,YoungJoo;Lekhanont,Kaevalin;Zhang,Cheng;Lee,Woo-Seok;Cano,Marisol;Bucala,Richard;Chuck,RoyS
目的：研究巨噬细胞移动抑制因子(MIF)在小鼠泪腺中的表达。方法：采用基因芯片技术对3只8周龄雌性CBA/J小鼠的6个泪腺组织进行分析。OMM-011，SuperArray Bioscience)和MIF的免疫荧光染色。对8周龄CBA/J小鼠的9个泪腺、8周龄C57BL/6小鼠的9个泪腺和8周龄BALB/c小鼠的7个泪腺进行了实时定量逆转录聚合酶链式反应。对5只8周龄雌性CBA/J小鼠的5个脾、5个肝脏和5个血清样本进行了酶联免疫吸附分析。结果：基因芯片分析显示MIF在小鼠泪腺中高表达。泪腺泡细胞呈强烈的MIF抗体染色。实时定量逆转录聚合酶链式反应显示，对照β-肌动蛋白mRNA的表达，CBA/J小鼠泪腺中MIF mRNA的表达水平低于C57BL/6和BALB/c小鼠。结论：小鼠泪腺表达高水平的巨噬细胞MIF，未见泪腺炎症的迹象。
Purpose:To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands.Methods:Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF. Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay.Results:Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription-polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of β-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera.Conclusion:The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.