Comparative interactomics provides evidence for functional specialization of the nuclear pore complex.
Comparative interactomics provides evidence for functional specialization of the nuclear pore complex.
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比较相互作用组学为核孔复合体的功能特化提供了证据。
DOI：
10.1080/19491034.2017.1313936
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Obado SO
中科院分区：
文献类型：
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作者：
Obado SO
真核细胞的核心结构是在十亿多年前建立起来的，并且在所有现存的谱系中基本上保留下来。然而，真核细胞也具有谱系特异性特征，通常与特定的功能要求相关。核孔复合物（NPC）是真核生物的核心结构之一，负责调节细胞核和细胞质之间的大分子交换，并充当核组织中心。NPC结构在一个真核生物超群中得到了最好的记录，即后鞭毛体（例如Saccharomyces grainaeandHomo sapiens），尽管它们在组成上相似，但在某些NPC亚复合体结构中有显着的变化。然而，NPC结构在真核生物界其他类群中的变化仍然知之甚少。我们探索了锥虫，高度分化的生物体，并映射和分配其NPC蛋白质的特定亚结构，以揭示其NPC架构。我们发现NPC中心结构支架是保守的，可能在所有真核生物中都是如此，但更多的外围元件可以表现出非常显著的谱系特异性损失，复制或其组分的其他改变。令人惊讶的是，锥虫缺乏mRNA输出平台的主要成分，这些成分不对称地定位在酵母和脊椎动物NPC中。与此同时，锥虫NPC几乎是完全对称的，核篮是不对称的唯一主要来源。我们认为这些特征指向NPC的逐步进化，其中涂层支架首先稳定孔，然后选择性门控出现并扩大，导致孔的核质和细胞质侧增加外周重塑机制。
The core architecture of the eukaryotic cell was established well over one billion years ago, and is largely retained in all extant lineages. However, eukaryotic cells also possess lineage-specific features, frequently keyed to specific functional requirements. One quintessential core eukaryotic structure is the nuclear pore complex (NPC), responsible for regulating exchange of macromolecules between the nucleus and cytoplasm as well as acting as a nuclear organizational hub. NPC architecture has been best documented in one eukaryotic supergroup, the Opisthokonts (e.g.Saccharomyces cerevisiaeandHomo sapiens), which although compositionally similar, have significant variations in certain NPC subcomplex structures. The variation of NPC structure across other taxa in the eukaryotic kingdom however, remains poorly understood. We explored trypanosomes, highly divergent organisms, and mapped and assigned their NPC proteins to specific substructures to reveal their NPC architecture. We showed that the NPC central structural scaffold is conserved, likely across all eukaryotes, but more peripheral elements can exhibit very significant lineage-specific losses, duplications or other alterations in their components. Amazingly, trypanosomes lack the major components of the mRNA export platform that are asymmetrically localized within yeast and vertebrate NPCs. Concomitant with this, the trypanosome NPC is ALMOST completely symmetric with the nuclear basket being the only major source of asymmetry. We suggest these features point toward a stepwise evolution of the NPC in which a coating scaffold first stabilized the pore after which selective gating emerged and expanded, leading to the addition of peripheral remodeling machineries on the nucleoplasmic and cytoplasmic sides of the pore.