Apolipoprotein E polymorphism in the Tunisian population:: Frequency and effect on lipid parameters
Apolipoprotein E polymorphism in the Tunisian population:: Frequency and effect on lipid parameters
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DOI：
10.1016/j.clinbiochem.2006.04.018
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发表时间：
2006-08-01
影响因子：
2.8
通讯作者：
Kaabachi, N.
中科院分区：
文献类型：
--
作者：
Jemaa, R.;Elasmi, M.;Kaabachi, N.
目的：我们确定载脂蛋白E（载脂蛋白E）基因等位基因的频率，并检查载脂蛋白E多态性和血脂参数之间的联系在突尼斯population.Design和方法：载脂蛋白E多态性进行了调查，并在122名男性和111名女性年龄在35至87岁的血脂参数进行了测量。7.3%，103：84.6%，104：8.1%。载脂蛋白E基因多态性与总胆固醇、载脂蛋白B和低密度脂蛋白胆固醇在男性和女性中的差异有显著性（P < 0.001）。等位基因102携带者的平均总胆固醇、载脂蛋白B和LDL-C浓度最低，而等位基因104携带者的水平最高。甘油三酯水平随等位基因的增加而增加，但没有达到统计学显著性。这些结果在调整了年龄、体重指数、性别、高血压、糖尿病和吸烟后保持不变。在肥胖人群中（BMI > 30 kg/M-2），ApoE 3等位基因纯合子的TG浓度显著低于ApoE 2或ApoE 4等位基因纯合子的TG浓度。结论：在突尼斯人群中，ApoE基因等位基因的分布与ApoE 4等位基因低患病率的南欧人群相似。载脂蛋白E基因的变异在决定血浆脂质水平中起作用。这些数据还表明，载脂蛋白E等位基因对血脂水平的影响部分取决于环境变量，如BMI。这些发现强调了基因/环境相互作用对肥胖对心血管危险因素的有害影响的重要性。(c)2006年加拿大临床化学家协会。All rights reserved.
Objectives: We determined the frequencies of apolipoprotein E (apo E) gene alleles and examined the association between apo E polymorphism and lipid parameters in a sample of the Tunisian population.Design and methods: Apo E polymorphism was investigated using PCR, and plasma lipid parameters were measured in 122 men and 111 women aged 35 to 87 years.Results: The allele frequencies were epsilon 2: 7.3%, epsilon 3: 84.6%, and epsilon 4: 8.1%. Apo E polymorphism was associated with significant differences (P < 0.001) in total cholesterol, apo B and LDL cholesterol in both men and women. epsilon 2 carriers had the lowest mean total cholesterol, apo B and LDL-C concentrations, and subjects with the epsilon 4 allele had the highest levels. Triglycerides levels increased with the epsilon 4 allele, but this did not reach statistical significance. These results remained unchanged after adjustment for age, body mass index, sex, hypertension, diabetes and smoking. However, in obese subjects (BMI > 30 k g/M-2), TG concentrations were significantly lower in individuals homozygous for the epsilon 3 allele compared to those with the alleles epsilon 2 or epsilon 4.Conclusion: In this sample of the Tunisian population, the distribution of apo E gene alleles is similar to that observed in Southern European populations with low prevalence of the epsilon 4 allele. Variations in the apo E gene play a role in determining plasma lipid levels. These data also suggest that effects of apo E alleles on lipids levels are partly dependent on environmental variables such as BMI. These findings highlight the importance of the gene/environment interaction on the deleterious effect of obesity on cardiovascular risk factors. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.