γ-H2AX and Phosphorylated ATM Focus Formation in Cancer Cells after Laser Plasma X Irradiation
γ-H2AX and Phosphorylated ATM Focus Formation in Cancer Cells after Laser Plasma X Irradiation
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DOI：
10.1667/rr2178.1
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发表时间：
2010-10-01
影响因子：
3.4
通讯作者：
Nishimura, Hiroaki
中科院分区：
文献类型：
--
作者：
Sato, Katsutoshi;Nishikino, Masaharu;Nishimura, Hiroaki
研究了激光等离子体X射线脉冲在医学和辐射生物学研究中的用途，并将激光等离子体X射线的效果与直线加速器等传统光源的效果进行了比较。研制了一种细胞辐照系统，利用超短高强度激光产生的铜k α (8 keV)线产生等离子体。用Gafchromic (R) EBT薄膜准确地估计了8kev激光等离子体x射线脉冲的吸收剂量。用约2 Gy的激光等离子体X射线照射细胞时，可以清晰地识别出γ - h2ax阳性细胞上的圆形区域。此外，8 keV激光等离子体X射线诱导的γ - h2ax和磷酸化的ataxia毛细血管扩张突变（ATM）病灶数量与4 MV X射线诱导的相当。这些结果表明，激光等离子体X射线源可用于辐射生物学的研究。(C) 2010年由辐射研究学会出版
The usefulness of laser plasma X-ray pulses for medical and radiation biological studies was investigated, and the effects of laser plasma X rays were compared with those of conventional sources such as a linear accelerator. A cell irradiation system was developed that used copper-K alpha (8 keV) lines from an ultrashort high-intensity laser to produce plasma. The absorbed dose of the 8 keV laser plasma X-ray pulse was estimated accurately with Gafchromic (R) EBT film. When the cells were irradiated with approximately 2 Gy of laser plasma X rays, the circular regions on gamma-H2AX-positive cells could be clearly identified. Moreover, the numbers of gamma-H2AX and phosphorylated ataxia telangiectasia mutated (ATM) foci induced by 8 keV laser plasma X rays were comparable to those induced by 4 MV X rays. These results indicate that the laser plasma X ray source may be useful for radiation biology studies. (C) 2010 by Radiation Research Society