An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus.
An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus.
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DOI：
10.1038/srep25873
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发表时间：
2016-05-13
影响因子：
4.6
通讯作者：
Tomonaga K
中科院分区：
文献类型：
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作者：
Horie M;Kobayashi Y;Honda T;Fujino K;Akasaka T;Kohl C;Wibbelt G;Mühldorfer K;Kurth A;Müller MA;Corman VM;Gillich N;Suzuki Y;Schwemmle M;Tomonaga K
内源性博尔纳病毒样L（EBLL）元件是来源于古老博尔纳病毒L基因的可遗传序列，其编码许多真核生物基因组中的病毒RNA依赖性RNA聚合酶（RdRp）。在这里，我们证明，蝙蝠属Eptesicus保存了超过1180万年的EBLL元素命名为eEBLL-1，它有一个完整的开放阅读框架的1,718个密码子。eEBLL-1编码序列显示，单负性病毒RdRp活性所必需的功能基序在EBLL-1基因中非常保守。遗传分析表明，在Eptesicus的进化过程中，自然选择对eEBLL-1起作用。值得注意的是，我们检测到有效的转录eEBLL-1的组织中的蝙蝠。据我们所知，这项研究是第一份报告表明，真核基因组已经获得了一个核糖核酸聚合酶基因从一个古老的病毒，有可能编码一个功能性的RdRp。
Endogenous bornavirus-like L (EBLL) elements are inheritable sequences derived from ancient bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in many eukaryotic genomes. Here, we demonstrate that bats of the genus Eptesicus have preserved for more than 11.8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of 1,718 codons. The eEBLL-1 coding sequence revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analyses showed that natural selection operated on eEBLL-1 during the evolution of Eptesicus. Notably, we detected efficient transcription of eEBLL-1 in tissues from Eptesicus bats. To the best of our knowledge, this study is the first report showing that the eukaryotic genome has gained a riboviral polymerase gene from an ancient virus that has the potential to encode a functional RdRp.