Tomato (Solanum lycopersicum) MADS-box transcription factor SlMBP8 regulates drought, salt tolerance and stress-related genes
Tomato (Solanum lycopersicum) MADS-box transcription factor SlMBP8 regulates drought, salt tolerance and stress-related genes
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番茄（Solanum lycopersicum）MADS-box转录因子SlMBP8调节干旱、耐盐和胁迫相关基因
DOI：
10.1007/s10725-017-0283-2
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发表时间：
2017
影响因子：
4.2
通讯作者：
Chen Guoping
中科院分区：
文献类型：
--
作者：
Yin Wencheng;Hu Zongli;Hu Jingtao;Zhu Zhiguo;Yu Xiaohui;Cui Baolu;Chen Guoping
干旱、高盐度和极端温度等不利的环境条件严重影响作物的生长和产量。MADS盒转录因子已被描述为参与应激反应。本研究从番茄中克隆了一个MADS盒转录因子基因SlMBP 8。甲基茉莉酸（MeJA）、高盐、高温、创伤和脱水均能诱导SlMBP 8的表达。脱落酸（阿坝）、1-氨基环丙烷-1-羧酸（ACC）和吲哚-3-乙酸（IAA）对SlMBP 8的转录有下调作用。为了进一步阐明SlMBP 8基因在非生物胁迫应答中的功能，以通过RNA干扰（RNAi）技术敲低SlMBP 8基因的番茄植株为材料，研究了干旱和盐胁迫对野生型（WT）和SlMBP 8-RNAi株系番茄幼苗的影响。在萌发后阶段，SlMBP 8-RNAi植物的幼苗生长受盐的抑制比WT小。转基因植株比野生型植株更耐干旱和盐胁迫，表现为叶绿素含量和含水量增加，失水率和丙二醛（MDA）含量降低。此外，在对照和非生物胁迫下，多个胁迫相关基因的表达在RNAi株系中显著上调。综上所述，这些结果表明SlMBP 8在干旱和高盐胁迫信号传导途径中作为负胁迫响应转录因子起作用，并且可能在耐旱和耐盐番茄的工程改造中具有有希望的应用。
Adverse environmental conditions, such as drought, high salinity and extreme temperature, severely affect the growth and productivity of crop plants. MADS-box transcription factors have been described to participate in stress responses. In our study, a MADS-box transcription factor gene,SlMBP8, has been cloned from tomato. The expression ofSlMBP8was induced by Methyl-jasmonic acid (MeJA), high salinity, high temperature, wounding and dehydration. Whereas, the transcript ofSlMBP8was down-regulated by Abscisic acid (ABA), 1-aminocyclopropane-1-carboxylic acid (ACC) and Indole-3-acetic acid (IAA). To further elucidate the function ofSlMBP8gene in response to abiotic stress, plants by knockdown ofSlMBP8through RNA interference (RNAi) were used for investigating the effect of drought and salt stresses on tomato seedlings of wild type (WT) andSlMBP8-RNAi lines. Seedling growth ofSlMBP8-RNAi plants was less inhibited by salt than WT at post-germination stage. Transgenic plants became more tolerant to drought and salt stress than WT plants in soil, which was demonstrated by higher levels of chlorophyll and water contents, lower water loss rate and malondialdehyde (MDA) contents. In addition, the expression of multiple stresses related genes were significantly up-regulated in the RNAi lines under control and abiotic stresses. Taken together, these results suggest that SlMBP8 function as a negative stress-responsive transcription factor in the drought and high salinity stress signaling pathways, and may have promising applications in the engineering of drought- and salt-tolerant tomato.