Monitoring changing toxigenicity of a cyanobacterial bloom by molecular methods
Monitoring changing toxigenicity of a cyanobacterial bloom by molecular methods
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DOI：
10.1128/aem.68.12.6070-6076.2002
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发表时间：
2002-12-01
影响因子：
4.4
通讯作者：
McKay, DB
中科院分区：
文献类型：
--
作者：
Baker, JA;Entsch, B;McKay, DB
蓝藻水华是供水水库潜在的健康危害。本文报道了用基于聚合酶链式反应的方法对一次蓝藻水华进行分析，以直接检测和鉴定存在的菌株并测定它们的毒性。来自澳大利亚新英格兰地区马尔帕斯大坝的一系列样本在2000年至2001年夏季的一次长时间的混合蓝藻水华期间进行了分析。马尔帕斯大坝过去曾被证明存在铜绿微囊藻的有毒水华，导致饮用该水库供水的人的肝脏受损。通过对藻蓝蛋白β亚基和α亚基基因之间的基因间隔区进行PCR扩增，可以在低细胞数下检测到蓝藻属。通过聚合酶链式反应扩增微囊藻毒素生物合成途径中的一个基因来确定微囊藻毒素的生产潜力。通过对卷曲鱼腥藻菌株的16S rRNA基因的一段区域的聚合酶链式反应(PCR)扩增，确定了产麻黄毒素的潜力。用小鼠生物测定法和高压液相色谱仪测定样品的毒性。我们表明，与显微镜和小鼠生物测定等其他方法相比，通过聚合酶链式反应可以更有效地识别和监测水华成分的致毒作用。我们还研究了鱼腥藻和微囊藻的产毒菌株。发生在这个地点，在开花的过程中，细胞类型和毒性发生了变化。这项工作表明，对潜在毒性的聚合酶链式反应检测可以加强对重大公共卫生危害的管理。
Cyanobacterial blooms are potential health hazards in water supply reservoirs. This paper reports analyses of a cyanobacterial bloom by use of PCR-based methods for direct detection and identification of strains present and determination of their toxigenicity. Serial samples from Malpas Dam, in the New England region of Australia, were analyzed during a prolonged, mixed cyanobacterial bloom in the summer of 2000 to 2001. Malpas Dam has been shown in the past to have toxic blooms of Microcystis aeruginosa that have caused liver damage in the human population drinking from this water supply reservoir. Cyanobacterial genera were detected at low cell numbers by PCR amplification of the phycocyanin intergenic spacer region between the genes for the beta and alpha subunits. The potential for microcystin production was determined by PCR amplification of a gene in the microcystin biosynthesis pathway. The potential for saxitoxin production was determined by PCR amplification of a region of the 16S rRNA gene of Anabaena circinalis strains. Toxicity of samples was established by mouse bioassay and high-pressure liquid chromatography. We show that bloom components can be identified and monitored for toxigenicity by PCR more effectively than by other methods such as microscopy and mouse bioassay. We also show that toxigenic strains of Anabaena and Microcystis spp. occur at this site and that, over the course of the bloom, the cell types and toxicity changed. This work demonstrates that PCR detection of potential toxicity can enhance the management of a significant public health hazard.