Aged adrenal medullary tissue survives intraocular grafting, forms nerve fibers and responds to nerve growth factor.
Aged adrenal medullary tissue survives intraocular grafting, forms nerve fibers and responds to nerve growth factor.
复制标题
老化的肾上腺髓质组织在眼内移植后存活下来，形成神经纤维并对神经生长因子产生反应。
DOI：
10.1002/jnr.490230206
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发表时间：
1989
影响因子：
4.2
通讯作者：
Ebendal,T
中科院分区：
文献类型：
--
作者：
Stromberg,I;Ebendal,T
将老龄大鼠（24个月大）和年轻成年大鼠（2个月大）的肾上腺髓质组织移植到先前交感神经切除的动物的前房眼。神经生长因子（NGF）每周双侧眼内注射。接枝后5周，虹膜作为整个载体制备，并进行Falck - Hillarp组织化学处理，以显示儿茶酚胺。NGF似乎在一定程度上阻止了年老和年轻移植物的体积减少。在NGF存在的情况下，老龄供体的移植物在宿主虹膜中形成了广泛、致密的纤维网络，与正常的肾上腺素能神经支配非常相似。未处理NGF的老年移植物的生长面积与处理NGF的相同，但密度较小。经NGF处理的年轻成人移植物的虹膜再神经支配与老年移植物的神经支配区域相似，但神经支配模式不规则，特别是靠近移植物。未经神经生长因子治疗的年轻成年捐赠者的移植在虹膜中产生了稀疏的、有限的神经网络。所有移植物在相同比例的细胞中都具有酪氨酸羟化酶、肾上腺素和多巴胺β羟化酶的免疫反应，但来自年轻供体的移植物体积较小。我们的结论是，在衰老的肾上腺中，染色质细胞向神经元表型转化、产生神经纤维和对外源性神经生长因子作出反应的能力是维持的。
Adrenal medullary tissue from aged (24 months old) and young adult (2 months old) rats was grafted to the anterior chamber of the eye of previously sympathectomized animals. Nerve growth factor (NGF) was administered by weekly bilateral intraocular injections. Five weeks postgrafting, irides were prepared as whole mounts and processed for Falck‐Hillarp histochemistry for visualization of catecholamines. NGF appeared to partially prevent the reduction in volume that both old and young grafts underwent. In the presence of NGF, an extensive, dense fiber network, closely resembling the normal adrenergic innervation, was formed in the host irides by grafts from aged donors. The area of outgrowth from aged transplants without NGF treatment was as large as with NGF treatment but less dense. The reinnervation of irides by NGF‐treated young adult grafts occupied a similar area as that seen with aged grafts, but the pattern of innervation was irregular, particularly close to the transplants. Transplants from young adult donors without NGF treatment generated a sparse, limited network of nerves in the irides. All grafts were tyrosine hydroxylase‐, adrenaline‐, and dopamine‐β‐hydroxylase‐immunoreactive in about the same proportion of cells, but the grafts from the young donors were smaller in size. We concluded that the ability of chromaffin cells to transform toward a neuronal phenotype, produce nerve fibers, and respond to exogenous NGF is maintained in aged adrenals.