RELATIONSHIP BETWEEN PRODUCTION AND RELEASE OF LYMPHOCYTE-ACTIVATING FACTOR (INTERLEUKIN-1) BY MURINE MACROPHAGES .1. EFFECTS OF VARIOUS AGENTS
RELATIONSHIP BETWEEN PRODUCTION AND RELEASE OF LYMPHOCYTE-ACTIVATING FACTOR (INTERLEUKIN-1) BY MURINE MACROPHAGES .1. EFFECTS OF VARIOUS AGENTS
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DOI：
10.1016/0008-8749(81)90481-0
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发表时间：
1981-01-01
影响因子：
4.3
通讯作者：
BARRANGER, JA
中科院分区：
文献类型：
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作者：
GERY, I;DAVIES, P;BARRANGER, JA
培养的小鼠腹膜巨噬细胞(M.vphi)产生和释放淋巴细胞激活因子(LAF)与释放白介素1(IL-1)的关系。被调查过了。未受刺激的vphi。在培养后的几个小时内产生高水平的细胞内LAF，但很少将这种活性释放到他们的培养基中。加入不同的促进剂可显著增加LAF的产生和释放，表现为3种不同的方式：乳胶珠使细胞内和细胞外的LAF活性均增加；脂多糖刺激细胞内LAF的释放明显增加，细胞外的LAF活性仅有轻微的增加；二氧化硅和葡萄糖脑苷(GL1)诱导细胞内LAF的释放急剧增加，细胞内的LAF活性略有增加。二氧化硅和GL1损伤培养的vphi，表现为乳酸脱氢酶释放的增加。二氧化硅和GL1增加了细胞内和细胞外LAF的水平，表明微囊藻的损伤。可能会刺激LAF的总产量。细菌脂多糖与硅胶或GL1联合作用对vphi有协同作用。释放非常高水平的LAF，远远超过个别制剂释放的水平。这些药物还在vphi分枝杆菌上进行了测试。经过预培养，以耗尽它们的LAF含量。乳胶、脂多糖或二氧化硅均能促进预培养的vphi产生和释放LAF，但均低于新鲜培养的vphi。LAF的释放水平并不一定反映培养的vphi产生LAF的总水平。并提示有害物质可能促进LAF的产生。
The relationship between the production and release of lymphocyte-activating factor (LAF, or interleukin 1) by cultured murine peritoneal macrophages (M.vphi.) was investigated. Unstimulated M.vphi. produce high levels of intracellular LAF within a few hours after culturing, but release little of this activity into their culture medium. Addition of various agents significantly increased the production and release of LAF, with 3 different patterns: both intracellular and extracellular LAF activities were increased in response to latex beads; a marked increase of intracellular LAF, with just a minimal elevation of extracellular activity, was stimulated by LPS; and sharp increases in LAF release, with small increments of the intracellular activity, were induced by silica and glucocerebroside (GL1). Silica and GL1 damaged the cultured M.vphi., as indicated by the increased release of lactate dehydrogenase. Silica and GL1 increased both intracellular and extracellular LAF levels, suggesting that damage of M.vphi. may stimulate total LAF production. A combination of LPS with silica or GL1 acted synergistically on M.vphi. to release very high levels of LAF, which far exceeded those released by the individual agents. The agents were also tested on M.vphi. which were precultured, to deplete their LAF content. Latex, LPS or silica increased LAF production and release by precultured M.vphi., but the levels were lower than those obtained with freshly cultured M.vphi.. The level of LAF release does not necessarily reflect the level of total LAF production by cultured M.vphi. and suggest that injurious agents may promote LAF production.