Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice
Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice
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DOI：
10.1016/j.fertnstert.2008.07.1733
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发表时间：
2009-09-01
影响因子：
6.7
通讯作者：
Lim, Jeong Mook
中科院分区：
文献类型：
--
作者：
Lee, Jae Hee;Lee, Eun Ju;Lim, Jeong Mook
目的：探讨白血病抑制因子（leukemia inhibitory factor， LIF）在胚胎干细胞（embryonic stem cells, ESCs）形成和自我更新中的必要性。设计：前瞻性动物模型研究。单位：韩国首尔国立大学配子与细胞生物技术实验室。实验动物：FI杂交B6D2FI小鼠。干预：在小鼠胚胎成纤维细胞（MEF）饲养器上培养囊胚内细胞团（ICM）细胞或在不含或含liff的培养基中维持市售ESCs。主要观察指标：细胞形态、LIF浓度、mRNA表达。结果：mef自身在无liff培养基中分泌146.5 ~ 175.3 pg/mL LIF。在MEF培养基上，ICM细胞形成了esc样菌落，E14和R1 ESCs在不含liff的培养基中成功维持。在无LIF的培养基中，介导LIF功能和调节严重度的基因的表达没有显著改变，内皮细胞的生长变化不明显，分化相关基因的mRNA表达和向胚状体的分化都没有改变。结论：在有成纤维细胞单层存在的培养基中，不需要在培养基中添加LIF来建立icm衍生的esc样菌落，并且建立的ESCs可以在无LIF的培养基中维持。[j] .中国农业科学（英文版），2009;32(2):444 - 444。(C)2009年美国生殖医学学会。)
Objective: To evaluate the necessity of leukemia inhibitory factor (LIF) in establishing and self-renewing embryonic stem cells (ESCs).Design: Prospective animal model Study.Setting: Gamete and Stern Cell Biotechnology Laboratory, Seoul National University, Korea.Animal(s): FI hybrid B6D2FI mice.Intervention(s): Inner cell mass (ICM) cells of blastocysts were cultured or commercially available ESCs were maintained in LIF-free or LIF-containing medium on mouse embryonic fibroblast (MEF) feeder.Main Outcome Measure(s): Cell morphology, LIF concentration, and mRNA expression.Result(s): The MEFs themselves secreted 146.5-175.3 pg/mL LIF in LIF-free medium. The ICM cells formed ESC-like colonies on MEF feeder, and E14 and R1 ESCs were successfully maintained in LIF-free medium. Expression of the genes either mediating LIF function or regulating sternness was not altered significantly, and change in the growth of ESCs was not prominent in LIF-free medium, Neither mRNA expression of differentiation-related genes nor differentiation into embryoid body was changed in the ESCs.Conclusion(s): Addition of LIF to culture medium is not necessary for establishing ICM-derived ESC-like colonies in the presence of fibroblast monolayer, and established ESCs can be maintained in an LIF-free medium. (Fertil Steril (R) 2009;92:1133-40. (C)2009 by American Society for Reproductive Medicine.)