Cultured human breast cancer cells lose selectivity in direct intercellular communication
Cultured human breast cancer cells lose selectivity in direct intercellular communication
复制标题
培养的人类乳腺癌细胞失去直接细胞间通讯的选择性
作者：
I. Fentiman;J. Taylor‐Papadimitriou
分子量高达1，600的各种小分子1可以直接在某些接触的动物细胞之间通过，可能是通过间隙连接2，3。这种通信可能部分介导多细胞生物和培养细胞的生长控制，因此正常通信模式的干扰可能与恶性生长有关4，5。基于代谢合作7的3 H-核苷酸转移方法6表明，通信并不总是一种全有或全无的现象，但可能是选择性的8，9。例如，来自良性肿瘤或乳汁的人乳腺上皮细胞不与人乳腺成纤维细胞（HumF）通信，尽管任一类型都可以与同源细胞良好地通信。我们研究了几种人类乳腺癌来源的癌细胞之间的通讯，发现与正常乳腺上皮不同，它们在通讯中没有选择性。癌细胞属于我们定义为非选择性通信者（与所有能够通信的细胞通信）或非通信者（不能与同源或异源细胞通信）的两个功能组中的一个或另一个，这表明恶性肿瘤的变化涉及细胞通信模式的一些变化。
VARIOUS small molecules, up to 1,600 molecular weight1, can pass directly between certain animal cells in contact, probably through gap junctions2,3. Such communication may partly mediate growth control in multicellular organisms and cultured cells, so that disturbances in the normal pattern of communication could be related to malignant growth4,5. The 3H-nucleotide transfer method6, based on metabolic cooperation7 has shown that communication is not always an all-or-none phenomenon, but may be selective8,9. For example, human mammary epithelial cells from benign tumours or milk do not communicate with human breast fibroblasts (HumF), although either type can communicate well with homologous cells. We have examined the communication between cancer cells from several human breast cancer sources and found that, unlike normal mammary epithelium, they do not show selectivity in communication. The cancer cells fall into one or other of the two functional groups we have defined as non-selective communicators (communicate with all cells capable of communication) or non-communicators (unable to communicate with homologous or heterologous cells) suggesting that the change to malignancy involves some change in the pattern of cell communication.