Effect of small coding genes on the circadian rhythms under elevated CO2 conditions in plants
Effect of small coding genes on the circadian rhythms under elevated CO2 conditions in plants
复制标题
高二氧化碳条件下小编码基因对植物昼夜节律的影响
DOI：
10.1007/s11103-020-01023-w
复制
发表时间：
2020
影响因子：
5.1
通讯作者：
Hanada Kousuke
中科院分区：
文献类型：
--
作者：
Higuchi-Takeuchi Mieko;Kondo Takayuki;Shimizu Minami;Kim You-Wang;Shinozaki Kazuo;Hanada Kousuke
大气中二氧化碳（CO2）的增加对植物的生长发育有显著的影响。为了探索二氧化碳升高的反应，我们在暴露于二氧化碳升高的拟南芥（arabidopsis thaliana）的一个时间过程（2小时- 14天）中生成了转录谱。在co2暴露升高的早中期，光合作用相关基因下调，昼夜节律相关基因异常调控。为了了解二氧化碳升高信号传导的新机制，我们重点研究了42个未知的小编码基因，这些基因在二氧化碳升高条件下表现出不同的表达模式。四株过表达小编码基因的转基因植物在二氧化碳浓度升高时表现出生长缺陷表型，而在当前二氧化碳浓度下则没有。转录组分析显示，在四种转基因植物中，昼夜节律相关基因普遍受到调控。这些与昼夜节律相关的基因是在叶片中二氧化碳浓度高的黑暗中转录的。综上所述，我们确定了四个小的编码基因可能参与了升高的二氧化碳信号传导到昼夜节律。
Increase in atmospheric carbon dioxide (CO2) has a significant effect on plant growth and development. To explore the elevated-CO2response, we generated transcriptional profiles over a time course (2 h–14 days) of exposure to elevated CO2inArabidopsis thaliana. Genes related to photosynthesis were down-regulated and circadian rhythm-related genes were abnormally regulated in the early to middle phase of elevated CO2exposure. To understand the novel mechanism of elevated CO2signaling, we focused on 42 unknown small coding genes that showed differential expression patterns under elevated CO2conditions. Four transgenic plants overexpressing the small coding gene exhibited a growth-defective phenotype under elevated CO2but not under current CO2. Transcriptome analysis showed that circadian rhythm-related genes were commonly regulated in four transgenic plants. These circadian rhythm-related genes were transcribed in the dark when CO2concentrations in the leaf was high. Taken together, our identified four small coding genes are likely to participate in elevated CO2signaling to the circadian rhythm.