Increase in macrophage migration inhibitory factor levels in lacrimal fluid of patients with severe atopic dermatitis
Increase in macrophage migration inhibitory factor levels in lacrimal fluid of patients with severe atopic dermatitis
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DOI：
10.1007/s00417-005-0168-3
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发表时间：
2006-07-01
影响因子：
2.7
通讯作者：
Ohno, Shigeaki
中科院分区：
文献类型：
--
作者：
Kitaichi, Nobuyoshi;Shimizu, Tadamichi;Ohno, Shigeaki
特应性皮炎是一种慢性炎症性皮肤病，通常涉及一些眼科特征。巨噬细胞移动抑制因子（MIF）是一种促炎细胞因子，与细胞介导的免疫反应的产生有关。虽然血清中的MIF水平可能会在严重的特应性皮炎升高，局部眼液中的MIF的数量仍然未知。我们检测了16例特应性皮炎患者、10例过敏性结膜炎患者和15例健康对照者泪液中的MIF水平。根据特应性皮炎评分（SCORAD）指数评价特应性皮炎的临床严重程度。通过对以下评分进行求和来计算该指数：程度标准、强度标准和主观症状。巨噬细胞移动抑制因子水平通过人MIF酶联免疫吸附试验测定。所有的比较都是双尾的，P值< 0.01被认为具有统计学意义，从健康对照受试者收集的泪液中的平均MIF浓度为0.69 +/- 0.2 ng/ml。中度至重度特应性皮炎患者的平均泪液MIF水平为17.87 ± 6.3 ng/ml（SCORAD >= 15，P=0.002），轻度特应性皮炎为0.93 +/- 0.08 ng/ml（SCORAD < 15），过敏性结膜炎为2.76 +/- 0.86 ng/ml（P=0.008）。在严重特应性皮炎的病例中，促炎细胞因子MIF水平在泪液和血清中升高。这些结果表明，MIF可能在诱导或增强与严重特应性皮炎相关的眼部特征方面发挥重要作用。
Atopic dermatitis is a chronic inflammatory skin disorder that often involves some ophthalmic features. Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that is associated with the generation of cell-mediated immune responses. Although serum MIF levels may be elevated in severe atopic dermatitis, the quantity of MIF in regional ocular fluid remains unknown. We measured MIF levels in tears (lacrimal fluid) of patients with atopic dermatitis.Tear samples were collected from 16 patients with atopic dermatitis, 10 patients with allergic conjunctivitis, and 15 healthy control subjects. The clinical severity of atopic dermatitis was evaluated according to the Scoring Atopic Dermatitis (SCORAD) index. The index was calculated by summing the following scores: extent criteria, intensity criteria, and subjective symptoms. Macrophage migration inhibitory factor levels were determined by a human MIF enzyme-linked immunosorbent assay. All comparisons were two-tailed, and P values < 0.01 were considered as statistically significant.The mean MIF concentration in lacrimal fluid collected from healthy control subjects was 0.69 +/- 0.2 ng/ml. The mean tear MIF levels were 17.87 +/- 6.3 ng/ml in moderate-to-severe atopic dermatitis (SCORAD >= 15, P=0.002), 0.93 +/- 0.08 ng/ml in mild atopic dermatitis (SCORAD < 15), and 2.76 +/- 0.86 ng/ml in allergic conjunctivitis (P=0.008).A proinflammatory cytokine MIF level was elevated in tears as well as serum in cases of severe atopic dermatitis. These results suggest that MIF may play an important role in the induction or enhancement of ophthalmic features related to severe atopic dermatitis.