Fabricating a pearl/PLGA composite scaffold by the low-temperature deposition manufacturing technique for bone tissue engineering
Fabricating a pearl/PLGA composite scaffold by the low-temperature deposition manufacturing technique for bone tissue engineering
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DOI：
10.1088/1758-5082/2/2/025002
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发表时间：
2010-06
期刊：
影响因子：
9
通讯作者：
Mingen Xu;Yanlei Li;H. Suo;Yongnian Yan;Li Liu;Qiujun Wang;Yakun Ge;Ying Xu
中科院分区：
文献类型：
--
作者：
Mingen Xu;Yanlei Li;H. Suo;Yongnian Yan;Li Liu;Qiujun Wang;Yakun Ge;Ying Xu
本文利用低温沉积法制备了珍珠/聚乳酸-聚乙醇酸(PEL/PLGA)复合支架。LDM技术可以在低温下保持生物材料的生物活性，同时制备出具有设计好的微观和宏观结构的支架。对珍珠粉、聚乳酸和1，4-二氧六环的混合物进行了工艺优化，制备了具有设计的分级结构的珍珠粉、聚乳酸和1，4-二氧六环的混合物，并在−40°C下冷冻干燥，根据三维模型制备了方形和指定骨形的支架。将骨髓干细胞种植在珍珠/PLGA支架上，然后在旋转细胞培养系统中培养。采用扫描电子显微镜、WST-1法、碱性磷酸酶活力测定、免疫荧光染色和实时逆转录聚合酶链式反应等方法检测MSCs向成骨细胞的黏附、增殖和分化情况。结果表明，该复合支架具有较高的孔隙率(81.98±3.75%)、合适的孔径(微孔：10µm；大孔：495±54µm)和力学性能(抗压强度：0.81±0.04 Mpa；弹性模量：23.14±0.75 Mpa)。与磷酸三钙/PLGA支架相比，珍珠/PLGA支架具有更好的生物相容性和骨传导性。这些结果表明珍珠/PLGA支架满足骨组织工程支架的基本要求。
Here we developed a composite scaffold of pearl/poly(lactic-co-glycolic acid) (pearl/PLGA) utilizing the low-temperature deposition manufacturing (LDM). LDM makes it possible to fabricate scaffolds with designed microstructure and macrostructure, while keeping the bioactivity of biomaterials by working at a low temperature. Process optimization was carried out to fabricate a mixture of pearl powder, PLGA and 1,4-dioxane with the designed hierarchical structures, and freeze-dried at a temperature of −40 °C. Scaffolds with square and designated bone shape were fabricated by following the 3D model. Marrow stem cells (MSCs) were seeded on the pearl/PLGA scaffold and then cultured in a rotating cell culture system. The adhesion, proliferation and differentiation of MSCs into osteoblasts were determined using scanning electronic microscopy, WST-1 assay, alkaline phosphatase activity assay, immunofluorescence staining and real-time reverse transcription polymerase chain reaction. The results showed that the composite scaffold had high porosity (81.98 ± 3.75%), proper pore size (micropores: <10 µm; macropore: 495 ± 54 µm) and mechanical property (compressive strength: 0.81 ± 0.04 MPa; elastic modulus: 23.14 ± 0.75 MPa). The pearl/PLGA scaffolds exhibited better biocompatibility and osteoconductivity compared with the tricalcium phosphate/PLGA scaffold. All these results indicate that the pearl/PLGA scaffolds fulfill the basic requirements of bone tissue engineering scaffold.