Correlation of denitrifying capability with the existence of nap, nir, nor and nos genes in diverse strains of soybean bradyrhizobia.
Correlation of denitrifying capability with the existence of nap, nir, nor and nos genes in diverse strains of soybean bradyrhizobia.
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DOI：
10.1264/jsme2.21.174
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发表时间：
2006-01-01
影响因子：
2.2
通讯作者：
Minamisawa, K.
中科院分区：
文献类型：
--
作者：
Sameshima-Saito, R.;Chiba, K.;Minamisawa, K.
为了阐明大豆慢生根瘤菌固氮能力多样性的遗传基础，比较了60株大豆慢生根瘤菌（Bradyrhizobium japonicum）和B. elkanii。结果表明，在大多数B菌株中，突变基因的存在直接决定了表型（终产物）。山茱萸利用重复序列指纹图和16 SrRNA基因序列分析系统发育位置，反映了该菌的遗传多样性和基因集。然而，具有高度重复序列（HRS）的B.根据RS-指纹图谱鉴定，日本血吸虫具有高度杂交，导致相关性不一致，可能是因为基因组重排。讨论了大豆慢生根瘤菌中固氮基因和能力的进化和生态学意义。
To clarify the genetic basis to the diversity of denitrifying ability in soyabean bradyrhizobia, we compared the end-products of denitrification (N2, N2O and NO2-) with the existence of denitrifying genes (napA, nirK, norCB and nosZ) of sixty phylogenetically diverse strains of Bradyrhizobium japonicum and B. elkanii. The results indicate that the existence of denitrifying genes directly determines phenotype (end-products) in most strains of B. japonicum. The denitrifying capability and gene set were reflected by phylogenetic position based on repeated sequences (RS)-fingerprints and 16S rRNA gene sequences. However, the denitrifying genes in HRS (highly repeated sequence-possessing) strains of B. japonicum, which were identified based on RS-fingerprints as having heavy hybridization, resulted in an inconsistent correlation probably because of genomic rearrangements. The evolutionary and ecological implications of the denitrifying genes and capability in soyabean bradyrhizobia are discussed.