Introgression the salinity tolerance QTLs Saltol into AS996, the elite rice variety of Vietnam.
Introgression the salinity tolerance QTLs Saltol into AS996, the elite rice variety of Vietnam.
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DOI：
10.4236/ajps.2012.37116
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Ham, L. H.
中科院分区：
文献类型：
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作者：
Huyen, L. T. N.;Cuc, L. M.;Ham, L. H.
本研究旨在利用分子标记辅助回交(MABC)技术培育耐盐高产水稻新品系。对水稻12条染色体上的500个SSR标记进行亲本多态标记筛选。其中在Saltol区的52个引物与两个亲本品种进行了比对，筛选出了用于筛选Saltol区育种群体的多态引物。对于ASS996/FL478的每个回交世代，大约利用分布在12条染色体上的63个多态标记对500株植株进行了筛选。选择受体等位基因最高达89.06%和86.36%的BC1F1植株P284和P307进行回交。利用3株受体等位基因分别高达94.03%和93.18%的BC2F_1植株进行BC3F_1代选育。BC3F_1株系以P284-112-209为最佳，具有全部的受体等位基因和Saltol区。P307-305-21、P284-112-195、P284-112-198、P284-112-213等4个单株仅有1个座位杂合，位居第二(共63个标记，覆盖在12条染色体上)。这5株植物被选作Saltol-AS996导入结果的选育品系。具有100%供体品种遗传背景的育种品系BC4F1为培育适应气候变化的耐盐新品种ASS996-Saltol做好了准备。
This study focus on developing new salinity tolerance and high yielding rice lines, using markers assisted backrossing (MABC). Total of 500 SSR markers on 12 rice chromosomes were screened for parental polymorphic markers. Of which, 52 primers in the Saltol region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol region of the breeding populations. For each backcross generation of ASS996/FL478, approx. 500 plants were screened with 63 polymorphic markers distributed on 12 chromosomes. The two BC1F1 plants P284 and P307 which had the highest recipient alleles up to 89.06% and 86.36%, were chosen for the next backcrossing. Three BC2F1 plants with the recipient alleles up to 94.03% and 93.18% were used to develop BC3F1 generation. The best BC3F1 plant was P284-112-209 with all the recipient alleles and Saltol region. The four plants P307-305-21, P284-112-195, P284-112-198, P284-112-213 were the second ranking with only one loci heterozygous (applied 63 markers covered on 12 chromosomes). These five plants were chosen as the breeding lines for result of Saltol-AS996 introgression. The breeding line BC4F1 having 100% genetic background of donor variety is ready for develop new salinity tolerant variety ASS996-Saltol to cope with climate change.