Efficiency of reconstitution of immunoglobulin G from blood specimens dried on filter paper and utility in herpes simplex virus type-specific serology screening
Efficiency of reconstitution of immunoglobulin G from blood specimens dried on filter paper and utility in herpes simplex virus type-specific serology screening
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DOI：
10.1128/cdli.9.6.1338-1342.2002
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发表时间：
2002-11-01
期刊：
影响因子：
--
通讯作者：
Su, X
中科院分区：
文献类型：
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作者：
Hogrefe, WR;Ernst, C;Su, X
如果在滤纸上干燥的全血样品与所采用的血清学检测相容，则使用来自偏远地区的血清进行研究将大大方便。由于干燥的血液样本不需要立即冷藏，占用空间小，易于运输，因此可用于在实验室资源有限的地理位置评价单纯疱疹病毒I型（HSV-1）和HSV-2的血清阳性率。我们评估了干血样用于检测型特异性HSV抗体的实用性。发现使用从干燥血液样品洗脱的免疫球蛋白G（IgG）的效率与大多数相应血清样品中IgG浓度的测量一致。所有干燥血液样品的平均IgG浓度与相应血清的平均IgG浓度之比为1：29。当将1：29的比例应用于22对样本中的每一对时，其中19对样本中的干燥血液样本和相应血清样本中的浓度之间的偏差小于15%。对于从干燥血液洗脱的IgG，未检测到正或负偏倚。在成对的干燥血液和血清样品中确定HSV-1和HSV-2抗体的存在，并且在44对中的43对中未检测到HSV血清状态的差异。其中一对的血清状态不同，血清样本为HSV-1弱阳性，干血样结果不确定。HSV抗体的检测通常是一致的干燥血液样品冷冻保存超过1年或在室温下保存30天，虽然在少数样品中发现反应性降低。
The performance of studies using sera from remote locations is greatly facilitated if whole-blood samples dried on filter paper are shown to be compatible with the serologic assay being employed. Since dried blood samples do not require immediate refrigeration, occupy little space, and are easily transported, they may be used for evaluating the seroprevalence of herpes simplex virus type I (HSV-1) and HSV-2 in geographic locations where laboratory resources are limited. We evaluated the utility of dried blood samples for the detection of type-specific HSV antibodies. The efficiency of using immunoglobulin G (IgG) eluted from dried blood samples was found to be consistent with measurement of IgG concentrations in most corresponding serum samples. The ratio of the mean IgG concentration for all dried blood samples to the mean IgG concentration for the corresponding sera was 1:29. When the 1:29 ratio was applied to each of the 22 pairs of samples, there was a deviation of less than 15% between concentrations in the dried blood sample and in the corresponding serum sample in 19 of the pairs. No positive or negative bias was detected for the IgG eluted from dried blood. The presence of HSV-1 and HSV-2 antibodies was determined in the paired dried blood and serum samples, and no differences in the HSV serostatuses were detected for 43 of the 44 pairs. One pair's serostatus varied, with the serum sample being weakly positive for HSV-1 and the dried blood sample results being equivocal. The detection of HSV antibodies was generally consistent for dried blood samples stored frozen for over I year or at room temperature for 30 days, although decreased reactivities were found in a few samples.