Effect of hepatocyte growth factor on invasion of prostate cancer cell lines.
Effect of hepatocyte growth factor on invasion of prostate cancer cell lines.
复制标题
肝细胞生长因子对前列腺癌细胞系侵袭的影响。
DOI：
10.3892/or.10.4.1001
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
I. Saiki
中科院分区：
文献类型：
--
作者：
Y. Fujiuchi;O. Nagakawa;K. Murakami;H. Fuse;I. Saiki
肝细胞生长因子(HGF)被认为在各种类型细胞的有丝分裂、运动、血管生成、迁移和侵袭的调节中发挥重要作用，并通过c-met原癌基因编码的特异性膜受体发挥作用。然而，HGF对前列腺癌细胞侵袭的影响机制尚不清楚。我们通过重组基底膜(Matrigel)观察了HGF对PC-3和DU-145前列腺癌细胞侵袭、向纤维连接蛋白底物迁移、基质金属蛋白酶-1和-9、膜型-1-基质金属蛋白酶、尿激酶型纤溶酶原激活物及其受体的蛋白和mRNA表达的影响。HGF可增加前列腺癌细胞的Matrigel侵袭和半趋化迁移。此外，HGF还可促进这些细胞产生基质金属蛋白酶-1、-9、MT1-基质金属蛋白酶、u-PA和uPAR。这些结果提示，HGF可能通过增强细胞的运动能力，增加MMPs和u-PA的产生，从而增加前列腺癌细胞的侵袭力。
Hepatocyte growth factor (HGF) was suggested to play an important role in the regulation of mitogenesis, motogenesis, angiogenesis, migration and invasion for various types of cells, and acts through a specific membrane receptor encoded by c-met proto-oncogene. However, the mechanism of the effect of HGF on tumor invasion of prostate cancer cells remains unclear. We investigated the effect of HGF on the invasion of PC-3 and DU-145 prostate cancer cells through a reconstituted basement membrane (Matrigel), the haptotactic migration to fibronectin substrate, the expression of protein and mRNA for matrix metalloproteinases (MMP)-1 and -9, membrane-type 1-MMP (MT1-MMP), urokinase-type plasminogen activator (u-PA) and its receptor (uPAR). HGF increased both Matrigel invasion and haptotactic migration of prostate cancer cells. Furthermore, HGF also increased the production of MMP-1 and -9, MT1-MMP, u-PA and uPAR of these cells. These results suggested that HGF increased the invasive potential of prostate cancer cells probably through enhancement of cell motility and the production of MMPs and u-PA.