GLYCINE METABOLISM BY PSEUDOMONAS-AERUGINOSA - HYDROGEN-CYANIDE BIOSYNTHESIS
GLYCINE METABOLISM BY PSEUDOMONAS-AERUGINOSA - HYDROGEN-CYANIDE BIOSYNTHESIS
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DOI：
10.1128/jb.130.2.826-831.1977
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发表时间：
1977-01-01
影响因子：
3.2
通讯作者：
CASTRIC, PA
中科院分区：
文献类型：
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作者：
CASTRIC, PA
甘氨酸的存在会刺激铜绿假单胞菌在合成培养基中产生 HCN。蛋氨酸增强了这种刺激，但不能替代甘氨酸作为氰化的刺激剂。苏氨酸和苯丙氨酸是甘氨酸刺激 HCN 产生的有效替代品。甘氨酸、苏氨酸和丝氨酸是 HCN 的良好放射性同位素前体，但蛋氨酸和苯丙氨酸则不是。铜绿假单胞菌的细胞提取物将[14C]苏氨酸转化为[14C]甘氨酸。 H14CN 是用[1-14C]甘氨酸或[2-14C]甘氨酸的低稀释标记产生的，表明初级或次级甘氨酸代谢中的标记是随机的。当全细胞喂食[1,2-14C]甘氨酸时，氰化物和碳酸氢盐是观察到的唯一放射性细胞外产物。
HCN production by P. aeruginosa in a synthetic medium is stimulated by the presence of glycine. Methionine enhances this stimulation but will not substitute for glycine as a stimulator of cyanogenesis. Threonine and phenylalanine are effective substitutes for glycine in the stimulation of HCN production. Glycine, threonine and serine are good radioisotope precursors of HCN, but methionine and phenylalanine are not. Cell extracts of P. aeruginosa convert [14C]threonine to [14C]glycine. H14CN is produced with low dilution of label from [1-14C]glycine or [2-14C]glycine, indicating a randomization of label in the primary or secondary glycine metabolism. When whole cells were fed [1,2-14C]glycine, cyanide and bicarbonate were the only radioactive extracellular products observed.