Induction of jasmonate signalling regulators MaMYC2s and their physical interactions with MaICE1 in methyl jasmonate-induced chilling tolerance in banana fruit.
Induction of jasmonate signalling regulators MaMYC2s and their physical interactions with MaICE1 in methyl jasmonate-induced chilling tolerance in banana fruit.
复制标题
DOI：
10.1111/j.1365-3040.2012.02551.x
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Minglei Zhao;Jun-ning Wang;W. Shan;Jianguang Fan;Jian-fei Kuang;Ke-qiang Wu;Xueping Li;Wei-xin Chen-Wei-xin
中科院分区：
文献类型：
--
作者：
Minglei Zhao;Jun-ning Wang;W. Shan;Jianguang Fan;Jian-fei Kuang;Ke-qiang Wu;Xueping Li;Wei-xin Chen-Wei-xin
MYC 2是一种碱性螺旋-环-螺旋（bHLH）转录因子，是茉莉酸（JA）应答激活的关键调节因子。然而，MYC 2参与茉莉酸甲酯（MeJA）诱导的果实耐冷性的分子细节仍不清楚。在本工作中，两个MYC 2基因，MaMYC 2a和MaMYC 2b，和一个同源物的诱导物的C-重复序列结合因子（CBF）基因，MaICE 1的分离和其特征在于从香蕉果实。MaMYC 2和MaICE 1均定位于细胞核。此外，MaMYC 2的N端富含脯氨酸的结构域（PRD）和酸性结构域（AD）对MaMYC 2在酵母细胞中的转录激活起重要作用。与MaICE 1的组成型表达不同，MaMYC 2a和MaMYC 2b在冷藏期间在MeJA处理后被迅速诱导。此外，蛋白质-蛋白质相互作用分析证实MaMYC 2与MaICE 1相互作用。MeJA还显著诱导了ICE-CBF冷反应途径基因MaCBF 1、MaCBF 2、MaCOR 1、MaKIN 2、MaRD 2和MaRD 5的表达。总之，我们的工作提供了强有力的证据表明，MaMYC 2通过与MaICE 1的物理相互作用和可能的功能协调参与了MeJA诱导的香蕉果实耐冷性，揭示了ICE 1响应冷胁迫以及诱导耐冷性发展的新机制。
MYC2, a basic helix-loop-helix (bHLH) transcription factor, is a key regulator in the activation of jasmonate (JA) response. However, the molecular details of MYC2 involving in methyl jasmonate (MeJA)-induced chilling tolerance of fruit remain largely unclear. In the present work, two MYC2 genes, MaMYC2a and MaMYC2b, and one homolog of the inducer of the C-repeat-binding factor (CBF) gene, MaICE1 were isolated and characterized from banana fruit. MaMYC2s and MaICE1 were found to be all localized in the nucleus. In addition, the proline-rich domain (PRD) and the acidic domain (AD) in the N-terminus were important for the transcriptional activation of MaMYC2 in yeast cells. Unlike MaICE1's constitutive expression, MaMYC2a and MaMYC2b were induced rapidly following MeJA treatment during cold storage. Moreover, protein-protein interaction analysis confirmed that MaMYC2s interacted with MaICE1. The expression of ICE-CBF cold-responsive pathway genes including MaCBF1, MaCBF2, MaCOR1, MaKIN2, MaRD2 and MaRD5 was also significantly induced by MeJA. Taken together, our work provides strong evidence that MaMYC2 is involved in MeJA-induced chilling tolerance in banana fruit through physically interacting and likely functionally coordinating with MaICE1, revealing a novel mechanism for ICE1 in response to cold stress as well as during development of induced chilling tolerance.