Competition strategies for the decolorization of a textile-reactive dye with the white-rot fungi Trametes versicolor under non-sterile conditions
Competition strategies for the decolorization of a textile-reactive dye with the white-rot fungi Trametes versicolor under non-sterile conditions
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DOI：
10.1002/bit.10623
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发表时间：
2003-06-20
影响因子：
3.8
通讯作者：
Banit, S
中科院分区：
文献类型：
--
作者：
Libra, JA;Borchert, M;Banit, S
各种白腐菌可以在无菌条件下氧化纺织染料；然而，将它们用于处理含有纺织染料的废水的一个重要考虑是，在非无菌条件下是否可以实现类似程度的处理。研究了四种策略在非无菌培养中优化使用杂色曲霉处理含重氮纺织染料C.I.活性黑5(RB5)废水的潜力。设计了三种悬浮培养策略来提高给定接种量的杂色假单胞菌在悬浮培养中的脱色活性，这些策略基于其对低pH的耐受性(培养基中pH降低)、胞外酶的产生(仅使用悬浮酶)以及其不依赖于生长的产酶能力(培养基中的氮素限制)。结果表明，pH降至3不会抑制细菌的生长，而杂色假单胞菌的产酶停止。单独使用胞外酶可以使真菌生长过程与废水处理脱钩；然而，在非无菌条件下，酶悬浮液的酶活性迅速下降。限制培养基中的氮素以抑制细菌生长的策略与第四种策略一起具有潜力，即在有机固体上培养真菌，以产生在非无菌条件下进行脱色过程的接种剂。以杂色木霉为唯一底物，在无菌条件下实现了对RB5的高度脱色。脱色率取决于真菌接种量。(C)2003年威利期刊公司。
A variety of white-rot fungi can oxidize textile dyes under sterile conditions; however, an important consideration for their use in treating wastewater containing textile dyes is whether similar degrees of treatment can be achieved under non-sterile conditions. Four strategies were investigated for their potential in optimizing the use of the fungus Trametes versicolor in non-sterile culture for treating wastewater containing the diazo textile dye C.I. Reactive Black 5 (RB5). Three strategies with suspended culture were designed to increase the decolorization activity in suspended culture from a given amount of T. versicolor inoculum based on its tolerance of low pH (pH reduction in medium), production of extracellular enzymes (use of suspended enzymes alone), and its ability to produce enzymes independent of growth (nitrogen limitation in medium). The results showed that reduction of the medium pH to 3 did not suppress bacterial growth, while enzyme production by T. versicolor ceased. The use of the extracellular enzymes alone would allow the decoupling of the process of fungal growth from wastewater treatment; however, the enzyme activity of an enzyme suspension decreased rapidly under non-sterile conditions. The strategy of limiting nitrogen in the medium to suppress bacterial growth has potential together with the fourth strategy, the cultivation of fungi on organic solids to produce inocula for a decolorization process under non-sterile conditions. A high degree of decolorization of RB5 under non-sterile conditions was achieved with T. versicolor grown on grains as sole substrate. The rate of decolorization was dependent on the amount of fungal inoculum used. (C) 2003 Wiley Periodicals, Inc.