Development of a quantification assay for the cysts of the toxic dinoflagellate Alexandrium tamarense using real-time polymerase chain reaction
Development of a quantification assay for the cysts of the toxic dinoflagellate Alexandrium tamarense using real-time polymerase chain reaction
复制标题
DOI：
10.1111/j.1444-2906.2005.01055.x
复制
发表时间：
2005-10
影响因子：
1.9
通讯作者：
Ryoma Kamikawa;S. Hosoi-Tanabe;S. Nagai;S. Itakura;Y. Sako
中科院分区：
文献类型：
--
作者：
Ryoma Kamikawa;S. Hosoi-Tanabe;S. Nagai;S. Itakura;Y. Sako
有毒甲藻塔玛亚历山大藻的孢囊是导致麻痹性贝类中毒（PSP）的水华的种子种群。然而，它是不可能确定Alexandrum spp。囊肿的形态特征的基础上。在这项研究中，我们准备了A。本研究在实验室条件下通过有性接合对tamarense孢囊进行了研究，并开发了一种用于聚合酶链反应（PCR）检测的有效DNA提取方法。利用A. tamarense孢囊，建立了鉴别和定量的方法，显示了种特异性和高灵敏度。tamarense孢囊，使用实时PCR。该方法也能够检测和定量A. tamarense囊肿准确地与过量的A.通过接合试验制备了链霉菌（catenella（Whedon and Kofoid）Balech）。
The cysts of toxic dinoflagellate Alexandrium tamarense are the seed population for the bloom responsible for paralytic shellfish poisoning (PSP). However, it is impossible to identify the Alexandrium spp. cyst on the basis of morphological features. In this study, we prepared A. tamarense cysts by sexual conjugation in laboratory conditions and developed an efficient DNA extraction method for polymerase chain reaction (PCR) assay. Using the A. tamarense cysts, we established the identification and quantification method showing the species specificity and the high sensistivity for A. tamarense cysts using real-time PCR. This assay was also able to detect and quantify the A. tamarense cysts accurately when mixed with excess cysts of A. catenella (Whedon and Kofoid) Balech prepared by conjugation experiment.