Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells
Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells
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DOI：
10.1016/j.devcel.2017.10.017
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发表时间：
2017-11-06
影响因子：
11.8
通讯作者：
Robert, Stephanie
中科院分区：
文献类型：
--
作者：
Majda, Mateusz;Grones, Peter;Robert, Stephanie
气生植物器官的表皮被认为是限制生长的，因为它作为一个连续的承重层，抵抗张力。叶表皮含有拼图块形的路面细胞，其形状已被提出是亚细胞的膨胀率的变化，诱导局部屈曲事件的结果。奇怪的是，这种局部压缩屈曲不应该发生给定的拉伸应力在整个表皮。使用计算建模，我们表明，最简单的情况下，解释路面细胞形状的表皮下的张力必须涉及机械壁的不均匀性，并沿着相邻细胞之间的垂周路面细胞壁。结合遗传学，原子力显微镜和免疫标记，我们证明，连续的细胞壁确实表现出混合机械化学特性。这种生化壁不均匀性先于壁弯曲。总之，这为复杂植物细胞形状的产生提供了一种可能的机制。
The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result of subcellular variations in expansion rate that induce local buckling events. Paradoxically, such local compressive buckling should not occur given the tensile stresses across the epidermis. Using computational modeling, we show that the simplest scenario to explain pavement cell shapes within an epidermis under tension must involve mechanical wall heterogeneities across and along the anticlinal pavement cell walls between adjacent cells. Combining genetics, atomic force microscopy, and immunolabeling, we demonstrate that contiguous cell walls indeed exhibit hybrid mechanochemical properties. Such biochemical wall heterogeneities precede wall bending. Altogether, this provides a possible mechanism for the generation of complex plant cell shapes.