Orexin-A acts on the paraventricular nucleus of the midline thalamus to inhibit locomotor activity in rats
Orexin-A acts on the paraventricular nucleus of the midline thalamus to inhibit locomotor activity in rats
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Orexin-A 作用于丘脑中线室旁核抑制大鼠运动活动
DOI：
10.1016/j.pbb.2009.06.017
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发表时间：
2009-10-01
影响因子：
3.6
通讯作者：
Kirouac, Gilbert J.
中科院分区：
文献类型：
--
作者：
Li, Yonghui;Li, Sa;Kirouac, Gilbert J.
食欲素（下丘脑分泌素）是一种新型肽，已被证明在控制行为唤醒方面发挥作用。中线丘脑 (PVT) 的室旁核是大脑中受食欲素纤维支配最密集的区域之一。此外，PVT 向纹状体的伏隔核区域发送密集投射，参与运动调节。本研究的目的是确定显微注射食欲素 A (OXA) 或食欲素受体拮抗剂 SB334867 对吗啡初治和吗啡致敏大鼠的 PVT 油运动活动 (LA) 的影响。在PVT内或PVT附近显微注射OXA（3μg/500nl）可以抑制在新的和熟悉的环境中测试的大鼠以及对重复注射吗啡表现出行为敏感的大鼠的LA。相比之下，在任何测试情况下，显微注射 SB334867 对 LA 都没有影响。使用涉及基于实验者的行为学行为分析的方法；我们发现，在中线丘脑显微注射 OXA 会减少 LA，同时增加修饰和冻结的表达。这些结果表明 OXA 可以作用于 PVT 和中线丘脑以产生独立于 LA 的唤醒。 (C) 2009 Elsevier Inc. 保留所有权利。
Orexins (hypocretins) are novel peptides that have been shown to play a role in control of behavioral arousal. The paraventricular nucleus of the midline thalamus (PVT) is one area of the brain that is the most densely innervated by orexin fibers. In addition, the PVT sends a dense projection to the nucleus accumbens an area, of the striatum involved in the regulation of locomotion. This study was done to determine the effect of microinjections of orexin-A (OXA) or the orexin receptor antagonist SB334867 in the PVT oil locomotor activity (LA) in morphine-naive and morphine-sensitized rats. Microinjections of OXA (3 mu g/500 nl) in or near the PVT inhibited LA in rats tested in a novel and familiar environment as well as in rats expressing behavioral sensitization to repeated injections of morphine. In contrast, microinjections of SB334867 had no effect on LA in any of the test situations. Using an approach involving experimenter based analysis of ethological behaviors; we found that microinjections of OXA in the midline thalamus decreased LA while at the same time increasing the expression of grooming and freezing. These results suggest that OXA can act on the PVT and the midline thalamus to produce arousal independent of LA. (C) 2009 Elsevier Inc. All rights reserved.