Surface-linked liposomal antigen induces IgE selective unresponsiveness in a T-cell independent fashion.
Surface-linked liposomal antigen induces IgE selective unresponsiveness in a T-cell independent fashion.
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表面连接的脂质体抗原以不依赖于 T 细胞的方式诱导 IgE 选择性无反应。
DOI：
10.2174/1568008033340252
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
T. Uchida
中科院分区：
文献类型：
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作者：
T. Uchida
我们以前报道，表面连接的脂质体抗原诱导IgE选择性无反应性。即使采用不同的抗原与脂质体或脂质体与不同脂质组分的偶联方法，结果也是一致的。在一项旨在阐明表面偶联脂质体抗原诱导的IgE选择性无反应性机制的研究过程中，我们发现了一种调节IgE产生的替代方法，该方法独立于T细胞的活性。用OVA-脂质体缀合物免疫小鼠诱导IgE选择性无反应性，而没有明显的Th 1极化。白细胞介素12（IL-12）、IL-10和CD 8（+）T细胞均不参与调节。此外，用OVA-脂质体免疫的小鼠的CD 4（+）T细胞能够在用明矾吸附的OVA免疫的无胸腺裸鼠中诱导抗原特异性IgE合成。另一方面，用OVA-脂质体免疫受体小鼠不诱导抗OVA IgE产生，即使当转移用明矾吸附的OVA免疫的小鼠的CD 4（+）T细胞时。在二次免疫应答中，OVA-脂质体增强了抗OVA IgG抗体的产生，但没有持续的IgE产生，这表明脂质体抗原诱导的IgE选择性无反应性涉及对IgE的直接影响，但不涉及体内IgG转换。这些结果表明，一种替代机制的作用，一个不涉及T细胞，在调节IgE的合成，并提出的可能性，表面连接的脂质体抗原是潜在的适用于开发一种新的疫苗，诱导最少的IgE合成。此外，鉴于对过敏原的相对低的过敏反应和增加的抗原性，这种形式的抗原制剂将适用于过敏原免疫治疗。
We previously reported that surface-linked liposomal antigen induced IgE-selective unresponsiveness. The results were consistent even when different coupling procedures for antigen with liposomes, or for liposomes with different lipid components, were employed. During the course of an investigation intended to clarify the mechanism of IgE-selective unresponsiveness induced by surface-coupled liposomal antigens, we discovered an alternative approach to regulate the production of IgE, one that is independent of the activity of T-cells. Immunization of mice with OVA-liposome conjugates induced IgE- selective unresponsiveness without apparent Th1 polarization. Neither interleukin-12 (IL-12), IL-10, nor CD8(+) T-cells participated in the regulation. Further, CD4(+) T-cells of mice immunized with OVA-liposome were capable of inducing antigen-specific IgE synthesis in athymic nude mice immunized with alum-adsorbed OVA. On the other hand, immunization of the recipient mice with OVA-liposome did not induce anti-OVA IgE production, even when CD4(+) T-cells of mice immunized with alum-adsorbed OVA were transferred. In the secondary immune response, OVA-liposome enhanced anti-OVA IgG antibody production but not the ongoing IgE production, suggesting that the IgE-selective unresponsiveness induced by the liposomal antigen involved direct effects on IgE but not IgG switching in vivo. These results suggest the role of an alternative mechanism, one not involving T-cells, in the regulation of IgE synthesis, and raise the possibility that surface-linked liposomal antigen is potentially applicable for the development of a novel vaccine that induces the least IgE synthesis. Moreover, given the relatively low allergic response to and increased antigenicity of the allergen, this form of antigen preparation would be applicable to allergen immunotherapy.