Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions.
Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions.
复制标题
巨细胞病毒糖蛋白和细胞受体相互作用的分析。
DOI：
10.1007/978-1-0716-1111-1_10
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Chan,GaryC
中科院分区：
文献类型：
--
作者：
Mahmud,Jamil;Chan,GaryC
人巨细胞病毒（HCMV）进入宿主细胞是一个复杂的过程，涉及一系列病毒糖蛋白与多种宿主细胞表面受体之间的相互作用。大量的研究致力于鉴定这些糖蛋白和细胞受体的相互作用，因为HCMV的广泛细胞向性表明了控制病毒结合和渗透的高度调节但适应性强的过程。然而，破译病毒糖蛋白的初始结合和细胞受体激活事件仍然具有挑战性。病毒进入过程中受体丰度相对较低和/或与糖蛋白的相互作用、膜受体的疏水性以及活化受体的快速降解和回收使得对HCMV进入和由HCMV接合启动的细胞信号传导途径的分析变得复杂。宿主膜。在这里，我们描述了在我们的实验室和其他人使用的不同方法来分析HCMV糖蛋白和宿主细胞受体之间的相互作用，在病毒生命周期的进入阶段。
Human cytomegalovirus (HCMV) entry into host cells is a complex process involving interactions between an array of viral glycoproteins with multiple host cell surface receptors. A significant amount of research has been devoted toward identifying these glycoprotein and cellular receptor interactions as the broad cellular tropism of HCMV suggests a highly regulated yet adaptable process that controls viral binding and penetration. However, deciphering the initial binding and cellular receptor activation events by viral glycoproteins remains challenging. The relatively low abundance of receptors and/or interactions with glycoproteins during viral entry, the hydrophobicity of membrane receptors, and the rapid degradation and recycling of activated receptors have complicated the analysis of HCMV entry and the cellular signaling pathways initiated by HCMV engagement to the host membrane. Here, we describe the different methodologies used in our laboratory and others to analyze the interactions between HCMV glycoproteins and host cellular receptors during the entry stage of the viral life cycle.