Reconfiguration of the achene and receptacle metabolic networks during strawberry fruit development
Reconfiguration of the achene and receptacle metabolic networks during strawberry fruit development
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DOI：
10.1104/pp.108.120691
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发表时间：
2008-10-01
期刊：
影响因子：
7.4
通讯作者：
Aharoni, Asaph
中科院分区：
文献类型：
--
作者：
Fait, Aaron;Hanhineva, Kati;Aharoni, Asaph
草莓（Fragaria 3ananassa）果实的解剖学特征是瘦果位于果实的外部，是研究果实发育规律的优良品种。它可以为初级代谢和次级代谢之间的串扰提供模型，其作用在该过程中至关重要。以草莓品种Herut为试材，采用气相色谱-质谱联用技术和液相色谱-质谱联用技术，分析了果实成熟过程中瘦果和花托中初级和次级代谢产物的组成。这些分析的结果表明，初级和次级代谢的变化反映了器官和发育的特异性。例如，容器的特点是增加糖和它们的直接衍生物，而瘦果的特点是大量减少的碳和氮丰富的化合物的水平，与存储相关的代谢物（e。G.棉子糖）。此外，容器，并在较小程度上瘦果，表现出动态波动的水平和性质的次生代谢产物在整个成熟过程。在花托中，原花色素和黄酮醇衍生物的特点主要是早期发展阶段，而花青素丰富的成熟红色阶段;在瘦果中，鞣花单宁和黄酮类化合物丰富的早期和晚期发展，分别。基于相关性的网络分析表明，在早期发育过程中，两个器官的代谢基本上是协调的。尽管如此，瘦果内和代谢途径之间的连接程度较高。数据进行了讨论的背景下，目前的模型的初级和次级代谢的相互作用和不同的植物器官之间的代谢相互作用。
The anatomy of strawberry (Fragaria 3 ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. It can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in the process. By combining gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry with the aim of addressing the metabolic regulation underlying fruit seed development, we simultaneously analyzed the composition of primary and secondary metabolites, separately, in achene and receptacle during fruit ripening of strawberry cultivar Herut. The results from these analyses suggest that changes in primary and secondary metabolism reflect organ and developmental specificities. For instance, the receptacle was characterized by increases in sugars and their direct derivatives, while the achene was characterized by a major decrease in the levels of carbon- and nitrogen-rich compounds, with the exception of storage-related metabolites (e. g. raffinose). Furthermore, the receptacle, and to a lesser extent the achene, exhibited dynamic fluctuations in the levels and nature of secondary metabolites across the ripening process. In the receptacle, proanthocyanidins and flavonol derivatives characterized mainly early developmental stages, while anthocyanins were abundant in the mature red stage; in the achene, ellagitannin and flavonoids were abundant during early and late development, respectively. Correlation-based network analysis suggested that metabolism is substantially coordinated during early development in either organ. Nonetheless, a higher degree of connectivity within and between metabolic pathways was measured in the achenes. The data are discussed within the context of current models both of the interaction of primary and secondary metabolism and of the metabolic interaction between the different plant organs.