Validation of a device for fluorescence sensing of rare circulating cells with diffusive light in an optical flow phantom model.
Validation of a device for fluorescence sensing of rare circulating cells with diffusive light in an optical flow phantom model.
复制标题
在光流模型模型中验证利用漫射光对稀有循环细胞进行荧光传感的装置。
DOI：
10.1109/iembs.2011.6090071
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Niedre,MarkJ
中科院分区：
文献类型：
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作者：
Zettergren,Eric;Vickers,Dwayne;Runnels,Judith;Lin,CharlesP;Niedre,MarkJ
生物组织中稀有循环细胞的检测和定量是生物医学研究中的一个重要问题，在生物医学研究中有着广泛的应用。目前的方法通常包括提取血液样本和体外细胞计数，或使用基于显微镜的荧光体内流式细胞术。这项工作的目标是开发一种仪器，用于在漫射光下无创地枚举小动物中非常罕见的循环细胞，与目前的方法相比，灵敏度提高了几个数量级。在这项工作中，我们描述了我们的系统设计，并表明单个荧光微球可以在模拟体内条件的模拟肢体光流幻象中检测到，这些幻象具有不同的光学特性。此外，我们展示了使用荧光（Vybrant-DiD）标记的Jurkat和多发性骨髓瘤细胞的单细胞计数能力。正在进行的工作包括在小鼠体内测试和表征我们的系统。
Detection and quantification of rare circulating cells in biological tissues is an important problem and has many applications in biomedical research. Current methods normally involve extraction of blood samples and counting of cells ex vivo, or the use of microscopy-based fluorescence in vivo flow cytometry. The goal of this work is to develop an instrument for non-invasively enumerating very rare circulating cells in small animals with diffuse light with several orders of magnitude sensitivity improvement versus current approaches. In this work, we describe the design of our system and show that single, fluorescent microspheres can be detected in limb-mimicking optical flow phantoms with varying optical properties chosen to simulate in vivo conditions. Further, we demonstrate single cell counting capabilities using fluorescently (Vybrant-DiD) labeled Jurkat and Multiple Myeloma cells. Ongoing work includes in vivo testing and characterization of our system in mice.